The methanol extract and its ethyl acetate fraction (EAF) of Actaea acuminata (Wall. ex. Royle) H. Hara roots were reported to exhibit significant antianxiety, anticonvulsant and antidepressant activities, and mild sedative activity. But the constituents responsible for these activities have not been isolated. The present study was undertaken to isolate neuroprotective compounds of A. acuminata following bioactivity-guided-fractionation. The column chromatography of EAF and its sub-fractions led to the isolation of four phenolic compounds (bergenin, gallic acid, acetyl bergenin and racemic mixture of diacetyl bergenin), which were characterized by IR and NMR spectral analysis. All the compounds exhibited significant antianxiety and antidepressant activities with respect to control. The gallic acid and bergenin did not show anticonvulsant activity, whereas acetyl bergenin and racemic mixture of diacetyl bergenin exhibited significant anticonvulsant activity. Neuropharmacological activities of A. acuminata are attributed due to polyphenolic compounds. Scientific validation of traditional claims of A. acuminata has opened up roadmap of research for the development of CNS affecting lead molecules.

中文翻译：

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Actaea acuminata (Wall. ex Royle) H. Hara 根的神经保护成分

据报道，Actaea acuminata (Wall. ex. Royle) H. Hara 根的甲醇提取物及其乙酸乙酯级分 (EAF) 表现出显着的抗焦虑、抗惊厥和抗抑郁活性，以及​​温和的镇静活性。但负责这些活动的成员并未被孤立。本研究旨在在生物活性引导的分级分离后分离 A. acuminata 的神经保护化合物。EAF 及其子馏分的柱色谱分离出四种酚类化合物（bergenin、没食子酸、乙酰 bergenin 和二乙酰 bergenin 的外消旋混合物），并通过 IR 和 NMR 光谱分析对其进行表征。相对于对照，所有化合物都表现出显着的抗焦虑和抗抑郁活性。没食子酸和bergenin没有显示抗惊厥活性，而乙酰 bergenin 和二乙酰 bergenin 的外消旋混合物表现出显着的抗惊厥活性。A. acuminata 的神经药理活性归因于多酚化合物。对 A. acuminata 传统声明的科学验证为影响铅分子的 CNS 的发展开辟了研究路线图。