The contamination of water by heavy metals is a worldwide environmental problem. Phytoremediation and constructed wetlands have become increasingly popular as more sustainable and environmentally friendly techniques of removing heavy metals from the wastewater. This study, therefore, investigated the phytoremediation of nickel by Alocasia puber (A. puber) in a constructed wetlands (CW) microcosm. This study identified the optimum conditions for nickel (Ni) removal from wastewater using response surface methodology (RSM) with central composite design (CCD). Two operational variables were assessed: exposure time and initial Ni concentration. The optimum conditions for the maximum removal of Ni from water were an exposure time of 10 days and 99.76 mg/L initial Ni concentration. The results indicated that 95.6% removal was achieved under the optimized conditions, with a high correlation coefficient (R2 = 0.97) between the statistical model and the experimental data. Field emission scanning electron microscopy images showed anatomical changes in the A. puber samples due to Ni exposure, and transmission electron microscopy images revealed some internal damages in the A. puber, but visual Ni toxicity symptoms, such as necrosis and chlorosis, were not observed in the A. puber. This study demonstrated that A. puber planted in a constructed wetland microcosm was able to remediate wastewater contaminated with Ni.

中文翻译：

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响应面法优化海芋对镍的植物修复

重金属对水的污染是一个世界性的环境问题。植物修复和人工湿地越来越受欢迎，因为它是从废水中去除重金属的更可持续和环境友好的技术。因此，本研究调查了 Alocasia puber (A. puber) 在人工湿地 (CW) 缩影中对镍的植物修复。本研究使用响应面法 (RSM) 和中心复合设计 (CCD) 确定了从废水中去除镍 (Ni) 的最佳条件。评估了两个操作变量：暴露时间和初始镍浓度。从水中最大程度去除 Ni 的最佳条件是暴露时间为 10 天和初始 Ni 浓度为 99.76 mg/L。结果表明，95。在优化条件下实现了 6% 的去除率，统计模型与实验数据之间具有较高的相关系数（R2 = 0.97）。场发射扫描电子显微镜图像显示由于暴露于 Ni 导致 A. puber 样品的解剖学变化，透射电子显微镜图像显示 A. puber 内部有一些损伤，但没有观察到肉眼可见的 Ni 毒性症状，如坏死和萎黄病在 A. 青春期。该研究表明，种植在人工湿地缩影中的青春期 A. puber 能够修复受镍污染的废水。和透射电镜图像显示 A. puber 存在一些内部损伤，但在 A. puber 中未观察到肉眼可见的 Ni 毒性症状，如坏死和萎黄。该研究表明，种植在人工湿地缩影中的青春期 A. puber 能够修复受镍污染的废水。和透射电镜图像显示 A. puber 存在一些内部损伤，但在 A. puber 中未观察到肉眼可见的 Ni 毒性症状，如坏死和萎黄。该研究表明，种植在人工湿地缩影中的青春期 A. puber 能够修复受镍污染的废水。