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ultiplexed Immunosensor Based on the Amperometric Transduction for Monitoring of Marine Pollutants in Sea Water
Sensors ( IF 3.4 ) Pub Date : 2020-09-27 , DOI: 10.3390/s20195532
J.-Pablo Salvador , Klaudia Kopper , Andrea Miti , Ana Sanchis , M.-Pilar Marco

Environmental pollutants vigilance is one of the main problems that the aquaculture industry has to face with the objective to ensure the quality of their products and prevent entrance in the food chain that finally may arrive to the consumer. Contaminants such as hormones, antibiotics or biocides are especially relevant due to their toxicity, pharmacological effect or hormonal activity that can be considered harmful for the final consumer. The contaminants can be detected in the environment where the food is growing, and their concentration can be found (i.e., seawater) in the range of µg·L−1, ng·L−1 or even in lower concentrations. Thus, sensitive and selective methods for their monitoring are required to avoid their arrival in the food chain. Here, the development of a multiplexed amperometric biosensor is described, based on the use of specific antibodies to reach the necessary detectability to measure the targeted contaminants directly in seawater. The multiplexed immunosensor allows the detection of four relevant pollutants, such as el Irgarol 1051, sulfapyridine, chloramphenicol and estradiol, reaching an IC50 of 5.04 ± 0.29, 3.45 ± 0.29, 4.17 ± 0.44 and 5.94 ± 0.28 µg·L−1, directly measured in seawater.

中文翻译:

基于电流转导的多功能免疫传感器监测海水中的海洋污染物

保持环境污染物警惕是水产养殖业必须面对的主要问题之一,以确保其产品质量并防止进入最终可能到达消费者的食物链。诸如激素,抗生素或杀生物剂之类的污染物特别重要,因为它们的毒性,药理作用或激素活性可能被认为对最终消费者有害。可以在食物生长的环境中检测到污染物,并且可以发现它们的浓度(即海水)在µg·L -1,ng·L -1的范围内甚至更低的浓度。因此,需要敏感和选择性的监测方法来避免它们进入食物链。在此,基于使用特定抗体达到必要的可检测性以直接测量海水中的目标污染物的方法,描述了一种多安培电流传感器的开发。多重免疫传感器可直接检测四种相关污染物,例如el Irgarol 1051,磺胺吡啶,氯霉素和雌二醇,IC 50分别为5.04± 0.29、3.45±0.29、4.17 ±0.44和5.94±0.28 µg·L -1。在海水中测量。
更新日期:2020-09-28
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