ABSTRACT

Background: Neurocysticercosis (NCC) is an acquired infection of central nervous system associated with epileptic seizures. The parasite ‘Taenia solium’ causes this disease and has a complex life cycle and molts into various stages that influence the host-parasite interaction. The disease has a long asymptomatic phase with viable cyst and degeneration of cyst and leaking cyst fluid has been associated with symptomatic phase. The parasite proteome holds the answers and clues to this complex clinical presentation and hence unraveling of proteome of parasite antigens is needed for better understanding of host-parasite interactions.

Objective: To understand the proteome make-up of T. solium cyst vesicular fluid (VF) and excretory secretory proteins (ESPs).

Methodology: The VF and ESPs for the study were prepared from cyst harvested from naturally infected swine. The samples were prepared for nano LC-MS by in-tube digestion of proteins. The spectra obtained were annotated and enrichment analysis was performed and in silico analysis was done.

Results: T. solium VF and ESPs have 206 and 247 proteins of varied make-up including pro-inflammatory and anti-inflammatory nature.

Conclusions: Due to varied make-up of VF and ESPs it can generate complex humoral and cellular immune response.

摘要

背景：神经囊尾蚴病（NCC）是一种与癫痫发作相关的中枢神经系统获得性感染。寄生虫“猪带绦虫”会导致这种疾病，并且具有复杂的生命周期和蜕皮到影响宿主与寄生虫相互作用的各个阶段。该病具有长期的无症状期，囊肿存活，囊肿变性和囊液渗漏与症状期有关。寄生虫蛋白质组为这种复杂的临床表现提供了答案和线索，因此需要阐明寄生虫抗原的蛋白质组，以便更好地了解宿主与寄生虫之间的相互作用。

目的：了解猪绦虫囊泡液 (VF) 和排泄分泌蛋白 (ESP)的蛋白质组组成。

方法：用于研究的 VF 和 ESP 是从从自然感染的猪身上收获的包囊制备的。通过蛋白质的管内消化制备用于纳米LC-MS的样品。对获得的光谱进行注释并进行富集分析并进行计算机分析。

结果： T. solium VF 和 ESP 具有 206 和 247 种不同组成的蛋白质，包括促炎和抗炎性质。

结论：由于 VF 和 ESP 的不同组成，它可以产生复杂的体液和细胞免疫反应。