Actin depolymerising factor (ADF) is an actin binding protein that is ubiquitous in animal and plant cells. It plays an important role in plant growth and development, as well as resistance to biotic and abiotic stress. The research of plant ADF family has been restricted to Arabidopsis thaliana (L.) Heynh. and some herb crops, but no woody cash crops have been reported to date. All members of the Cajanus cajan (L.) Millsp. ADF (CcADF) family were identified from the pigeon pea genome, and distributed among the four subfamilies by phylogenetic analysis. CcADFs were relatively conservative in gene structure evolution, protein structure and functional expression, and different CcADFs showed specific expression patterns under different treatments. The expression characteristics of several key CcADFs were revealed by analysing the stress response pattern of CcADFs and the time series RNA-seq of aluminium stress. Among them, CcADF9 in the first subgroup specifically responded to aluminium stress in the roots; CcADF3 in the second subgroup intensively responded to fungal infection in the leaves; and CcADF2 in the fourth subgroup positively responded to various stress treatments in different tissues. This study extended the relationship between plant ADF family and aluminium tolerance, as well as adding to the understanding of CcADF family in woody crops.

肌动蛋白解聚因子（ADF）是一种普遍存在于动物和植物细胞中的肌动蛋白结合蛋白。它在植物生长发育以及抵抗生物和非生物胁迫方面发挥着重要作用。植物ADF家族的研究仅限于拟南芥(Arabidopsis thaliana (L.) Heynh)。和一些草本作物，但迄今为止尚未有木本经济作物的报道。 Cajanus cajan (L.) Millsp 的所有成员。从木豆基因组中鉴定出ADF ( CcADF ) 家族，并通过系统发育分析将其分布在四个亚科中。 CcADF在基因结构进化、蛋白质结构和功能表达方面相对保守，不同的CcADF在不同处理下表现出特定的表达模式。通过分析CcADF的应激反应模式和铝胁迫的时间序列RNA-seq，揭示了几个关键CcADF的表达特征。其中，第一亚组中的CcADF9特异性响应根部的铝胁迫；第二个亚组中的CcADF3对叶子中的真菌感染有强烈反应；第四个亚组中的CcADF2对不同组织中的各种应激治疗有积极反应。这项研究扩展了植物ADF家族与铝耐受性之间的关系，并增加了对木本作物CcADF家族的了解。