Differential immune responses of immunoglobulin Z subclass members in antibacterial immunity in a zebrafish model,Immunology

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Differential immune responses of immunoglobulin Z subclass members in antibacterial immunity in a zebrafish model
Immunology ( IF 4.9 ) Pub Date : 2020-09-26 , DOI: 10.1111/imm.13269
Jian-Fei Ji ₁ , Chong-Bin Hu ₁ , Tong Shao ₁ , Dong-Dong Fan ₁ , Nan Zhang ₁ , Ai-Fu Lin ₁ , Li-Xin Xiang ₁ , Jian-Zhong Shao _{1,

2}

Affiliation

Immunoglobulin Z (IgZ) or its equivalent immunoglobulin T (IgT) is a newly identified immunoglobulin (Ig) class from teleost fish. This Ig class is characterized by its involvement in mucosa‐associated lymphoid tissues (MALTs) for mucosal defence against pathogen infection. Recently, several subclass members of IgZ/IgT, such as IgZ, IgZ2, Igτ1, Igτ2 and Igτ3, have been further identified from zebrafish, common carp and rainbow trout. However, the functional diversity and correlation among these subclasses remain uncertain. Here, we explored the differential immune reactions of the IgZ and IgZ2 subclasses in antibacterial immunity in a zebrafish model. IgZ was extensively distributed in the peripheral serum and skin/gill MALTs and showed a rapid induction upon bacterial infection. IgZ2 was specialized in skin/gill MALTs and showed a strong induction following IgZ production. Correspondingly, the IgZ⁺ B cells had a wider distribution in the systemic primary/secondary lymphoid tissues and MALTs than the IgZ2⁺ B cells, which were predominant in MALTs. IgZ and IgZ2 exhibited a complementary effect in antibacterial immunity by possessing differential abilities. That is, IgZ is preferentially involved in bactericidal reaction that is in part C1q‐dependent, and IgZ2 participates in neutralization action through bacteria‐coating activity. The production of IgZ largely depended on the αβ T/CD4⁺ T cells, whereas that of IgZ2 did not, suggesting the different dependencies of IgZ and IgZ2 on systemic immunity. Our findings demonstrate that the functional behaviour and mechanism of the IgZ/IgT family are more diverse than previously recognized and thus improve the current knowledge about this ancient Ig class.

中文翻译：

斑马鱼模型中免疫球蛋白 Z 亚类成员在抗菌免疫中的差异免疫反应

免疫球蛋白 Z (IgZ) 或其等效免疫球蛋白 T (IgT) 是从硬骨鱼中新鉴定出的一类免疫球蛋白 (Ig)。此类 Ig 的特点是参与粘膜相关淋巴组织 (MALT)，用于粘膜防御病原体感染。近年来，从斑马鱼、鲤鱼和虹鳟鱼中进一步鉴定出IgZ/IgT的几个亚类成员，如IgZ、IgZ2、Igτ1、Igτ2和Igτ3。然而，这些子类之间的功能多样性和相关性仍然不确定。在这里，我们在斑马鱼模型中探讨了 IgZ 和 IgZ2 亚类在抗菌免疫中的差异免疫反应。 IgZ 广泛分布于外周血清和皮肤/鳃 MALT 中，并且在细菌感染时表现出快速诱导。 IgZ2 专门针对皮肤/鳃 MALT，并在 IgZ 产生后表现出强烈的诱导作用。相应地，IgZ ⁺ B 细胞在全身初级/次级淋巴组织和 MALT 中的分布比在 MALT 中占主导地位的 IgZ2 ⁺ B 细胞更广泛。 IgZ和IgZ2具有不同的能力，在抗菌免疫方面表现出互补作用。也就是说，IgZ 优先参与部分依赖于 C1q 的杀菌反应，而 IgZ2 通过细菌包被活性参与中和作用。 IgZ 的产生很大程度上依赖于 αβ T/CD4 ⁺ T 细胞，而 IgZ2 的产生则不然，这表明 IgZ 和 IgZ2 对全身免疫的依赖性不同。我们的研究结果表明，IgZ/IgT 家族的功能行为和机制比之前认识的更加多样化，从而提高了目前对这一古老 Ig 类别的认识。

更新日期：2020-09-26

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