Focused oxidative stress of the specific organelles (e.g., endoplasmic reticulum (ER) and mitochondrion) of cancer cells can boost the immunogenic cell death (ICD) effect for cancer immunotherapy. Herein, an ER-targeted bioprobe with aggregation-induced emission (AIE) characteristics (TPE-PR-FFKDEL) was rationally designed and synthesized by integrating a new AIE photosensitizer with ER targeting peptide, which has been demonstrated to be able to efficiently induce ER oxidative stress to evoke ICD. Compared with the photosensitizer hypericin that is well-known as an ER-targeted ICD inducer, TPE-PR-FFKDEL can lead to more robust emission of immunostimulatory damage-associated molecular patterns such as surface-exposed cal-reticulin, ATP secretion, and high-mobility group protein B1 (HMGB1) and heat shock protein 70 (HSP 70) expression. Furthermore, a range of immune responses are activated to protect mice from the attack of cancer cells in vivo.

特定细胞器的集中氧化应激（例如癌细胞的内质网（ER）和线粒体（ER）可以增强针对癌症免疫疗法的免疫原性细胞死亡（ICD）效果。本文中，通过将新的AIE光敏剂与ER靶向肽相结合，合理设计并合成了具有凝集诱导发射（AIE）特征的ER靶向生物探针（TPE-PR-FFKDEL），这已被证明能够有效诱导ER氧化应激引起ICD。与众所周知作为ER靶向ICD诱导剂的光敏剂金丝桃素相比，TPE-PR-FFKDEL可以导致免疫刺激性损伤相关分子模式的更强发射，例如表面暴露的网状网状蛋白，ATP分泌和高运动组蛋白B1（HMGB1）和热休克蛋白70（HSP 70）的表达。此外，体内。