当前位置：
X-MOL 学术
›
Nano-Micro Lett.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Intracellular Delivery of mRNA in Adherent and Suspension Cells by Vapor Nanobubble Photoporation
Nano-Micro Letters ( IF 31.6 ) Pub Date : 2020-09-27 , DOI: 10.1007/s40820-020-00523-0 Laurens Raes 1, 2 , Stephan Stremersch 1, 2 , Juan C Fraire 1 , Toon Brans 1, 2 , Glenn Goetgeluk 2, 3 , Stijn De Munter 2, 3 , Lien Van Hoecke 2, 4, 5 , Rein Verbeke 1, 2 , Jelter Van Hoeck 1, 2 , Ranhua Xiong 1 , Xavier Saelens 4, 6 , Bart Vandekerckhove 2, 3 , Stefaan De Smedt 1, 2 , Koen Raemdonck 1, 2 , Kevin Braeckmans 1, 2
中文翻译：
通过蒸气纳米气泡光穿孔在贴壁细胞和悬浮细胞中进行 mRNA 的细胞内递送
更新日期：2020-09-28
Nano-Micro Letters ( IF 31.6 ) Pub Date : 2020-09-27 , DOI: 10.1007/s40820-020-00523-0 Laurens Raes 1, 2 , Stephan Stremersch 1, 2 , Juan C Fraire 1 , Toon Brans 1, 2 , Glenn Goetgeluk 2, 3 , Stijn De Munter 2, 3 , Lien Van Hoecke 2, 4, 5 , Rein Verbeke 1, 2 , Jelter Van Hoeck 1, 2 , Ranhua Xiong 1 , Xavier Saelens 4, 6 , Bart Vandekerckhove 2, 3 , Stefaan De Smedt 1, 2 , Koen Raemdonck 1, 2 , Kevin Braeckmans 1, 2
Affiliation
-
Vapor nanobubble (VNB) photoporation represents a promising physical technique for mRNA transfection of adherent and suspension cells.
-
A multitude of parameters related to the VNB photoporation procedure were optimized to enable efficient mRNA transfection.
-
VNB photoporation was found to yield five times more living, transfected Jurkat T cells as compared to electroporation, i.e., currently the standard nonviral transfection technique for T cells.
中文翻译：
通过蒸气纳米气泡光穿孔在贴壁细胞和悬浮细胞中进行 mRNA 的细胞内递送
-
蒸气纳米气泡 (VNB) 光穿孔代表了一种用于贴壁细胞和悬浮细胞 mRNA 转染的有前景的物理技术。 -
优化了与 VNB 光穿孔程序相关的多个参数，以实现有效的 mRNA 转染。 -
研究发现，与电穿孔（即目前 T 细胞的标准非病毒转染技术）相比，VNB 光穿孔产生的活转染 Jurkat T 细胞多五倍。
京公网安备 11010802027423号