In this work, we have used Stöber method to prepare 200 nm SiO₂ particles and then immobilized metal affinity ligand iminodiacetic acid (IDA) and charged them with Ni²⁺ ions. The particles show high absorption efficiency to bovine hemoglobin and the maximum extraction amounts reached 43.4 mg/g. The particles have been applied to separate a model protein mixture of bovine hemoglobin (BHb) and bovine serum albumin (BSA). They can be separated and showed low non-specific adsorption. Then we have used these particles to extract BHb from the mixture of BHb and DNA. They have potential applications in removing abundant protein in proteomic analysis, and we hope it may become a simple, convenient, and potentially effective way to purify hemoglobin in serum.

中文翻译：

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Ni2 +-官能化的二氧化硅纳米粒子对牛血红蛋白的选择性吸附

在这项工作中，我们已经使用Stöber方法制备了200 nm的SiO ₂颗粒，然后固定了金属亲和配体亚氨基二乙酸（IDA），并向其中加入了Ni ²⁺离子。该颗粒显示出对牛血红蛋白的高吸收效率，最大提取量达到43.4 mg / g。该颗粒已应用于分离牛血红蛋白（BHb）和牛血清白蛋白（BSA）的模型蛋白质混合物。它们可以被分离并显示出低的非特异性吸附。然后，我们使用这些颗粒从BHb和DNA的混合物中提取BHb。它们在蛋白质组学分析中去除大量蛋白质方面具有潜在的应用前景，我们希望它可能成为纯化血清中血红蛋白的简单，方便且潜在有效的方法。