Background: Three-dimensional (3D) printing using melt electrowriting (MEW) technology is a recently developed technique to produce biocompatible micron-level mesh scaffolds layer-by-layer that can be seeded with cells for tissue engineering. Examining cell behavior, such as growth rate and migration, can be problematic in these opaque 3D scaffolds. A straightforward and quantitative method was developed to examine these cellular parameters on poly-ɛ-caprolactone (PCL) multilayered MEW scaffolds developed as components of the annulus fibrosus region of bioengineered intervertebral discs.

中文翻译：

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使用 Sulforhodamine B 监测干细胞行为的熔化电写夹心支架技术

背景：使用熔体电写 (MEW) 技术的三维 (3D) 打印是最近开发的一种技术，可以逐层生产生物相容性微米级网状支架，可以接种细胞进行组织工程。在这些不透明的 3D 支架中检查细胞行为，例如生长速率和迁移，可能会出现问题。开发了一种直接和定量的方法来检查聚-ɛ-己内酯 (PCL) 多层 MEW 支架上的这些细胞参数，该支架是作为生物工程椎间盘纤维环区域的组成部分开发的。