The study was to formulate 2% neem and 2% liquorice mouthwashes and to compare the antimicrobial efficacy of these mouthwashes with the standard 0.2% chlorhexidine mouthwash. Alcoholic solution was prepared and added to neem mixture and liquorice mixture separately and made up to a volume of 16000 ml with purified water. Nine dilutions of each drug were done with Brain heart infusion broth (BHI) for MIC. Culture suspension was added in each serially diluted tube of 200 μl. The tubes were incubated for 24 hours and observed for turbidity. Minimum inhibitory concentration (MIC) of 2% neem, 2% liquorice and 0.2% chlorhexidine against Lactobacillus, Actinomyces naeslundii, Streptococcus sanguis, Streptococcus mutans is determined by serial dilution analysis. Streptococcus mutans shows sensitivity to all three mouthwashes at a concentration starting from 0.2 μg/ml. Lactobacillus shows sensitivity to neem and chlorhexidine mouthwashes at a concentration starting from 1.6 μg/ml, whereas liquorice is effective at a concentration starting from 3.125 μg/ml. Streptococcus sanguis shows sensitivity to chlorhexidine and liquorice mouthwashes at a concentration starting from 25 μg/ml, whereas it shows sensitivity to neem at a concentration starting from 50 μg/ml. Actinomyces naeslundii shows sensitivity to chlorhexidine and neem mouthwashes at a concentration starting from 1.6 μg/ml, whereas it shows sensitivity to liquorice at a concentration starting from 3.125 μg/ml. Analysis showed an inhibition of all the four strains by the mouthwashes. The MIC for the studied mouthwashes was found to be similar to that of 0.2% chlorhexidine.

中文翻译：

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印he和甘草素与洗必泰对血链球菌，变形链球菌，乳酸杆菌和内氏放线菌的体外抗菌研究–体外研究

该研究旨在配制2％印em和2％甘草精漱口水，并将这些漱口水与标准0.2％洗必泰漱口水的抗菌功效进行比较。制备酒精溶液，并将其分别加入印mixture混合物和甘草混合物中，并用纯净水补足至16000 ml的体积。每种药物的九种稀释液均用用于MIC的脑心浸液（BHI）进行。将培养悬浮液添加到每个200μl的连续稀释试管中。将试管孵育24小时，并观察其浊度。通过系列稀释分析确定了2％印em，2％甘草精和0.2％洗必泰对乳杆菌，内生放线菌，血链球菌，变形链球菌的最小抑制浓度（MIC）。变形链球菌在浓度从0.2μg/ ml开始的所有三种漱口液中均显示出敏感性。乳杆菌对浓度为1.6μg/ ml起的印ne和洗必泰漱口液具有敏感性，而甘草精对浓度为3.125μg/ ml起有效。血链球菌对浓度从25μg/ ml开始的洗必泰和甘草漱口水显示敏感性，而对浓度从50μg/ ml开始的印ne敏感。Naeslundii放线菌对浓度为1.6μg/ ml的洗必泰和印em漱口水显示敏感性，而对浓度为3.125μg/ ml的甘草糖显示敏感性。分析显示漱口水可抑制所有四种菌株。