A personal glucose meter (PGM)-based method for quantitative detection of a urinary nucleic acid biomarker in prostate cancer screening, the so-called PCA3, is reported herein. A sandwich-type genoassay is conducted on magnetic beads to collect the target from the sample by specific hybridization, making the assay appropriate for PCA3 detection in biological fluids. The success of the method hinges on the use of alkaline phosphatase (ALP) to link the amount of nucleic acid biomarker to the generation of glucose. In particular, specifically attached ALP molecules hydrolyze D-glucose-1-phosphate into D-glucose, thus enabling the amplification of the recorded signal on the personal glucose meter. The developed genoassay exhibits good sensitivity (3.3 ± 0.2 mg glucose dL⁻¹ pM⁻¹) for PCA3, with a dynamic range of 5 to 100 pM and a quantification limit of 5 pM. Likewise, it facilitates point-of-care testing of nucleic acid biomarkers by using off-the-shelf PGM instead of complex instrumentation involved in traditional laboratory-based tests.

中文翻译：

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用于前列腺癌筛查的核酸生物标志物检测


本文报道了一种基于个人血糖仪（PGM）的方法，用于在前列腺癌筛查中定量检测尿核酸生物标志物，即所谓的PCA3。在磁珠上进行夹心型基因测定，通过特异性杂交从样品中收集靶标，使该测定适合生物液体中的 PCA3 检测。该方法的成功取决于使用碱性磷酸酶（ALP）将核酸生物标志物的量与葡萄糖的产生联系起来。特别是，特定附着的 ALP 分子将 D-葡萄糖-1-磷酸水解为 D-葡萄糖，从而能够放大个人血糖仪上记录的信号。开发的基因测定法对 PCA3 表现出良好的灵敏度（3.3 ± 0.2 mg 葡萄糖 dL ^-1 pM ^-1 ），动态范围为 5 至 100 pM，定量限为 5 pM。同样，它通过使用现成的 PGM 而不是传统实验室测试中涉及的复杂仪器，促进了核酸生物标志物的现场检测。