Abstract

In the present study, sodium alginate (SA):polyvinyl alcohol(PVA) blends with different concentrations of cardamom extract (CE) were electrospun. The SEM images show that the optimum content of CE was 10 wt% resulting in fibers with average diameter of 233 ± 33 nm. FTIR spectra was employed to investigate probable interactions between polymers and CE. The CE release follows first order kinetics with 86% initial burst release at the first 24 h necessary to desired antibacterial activity. To investigate the biological properties, MTT assay, cell adhesion and antibacterial test were carried out. The SA:PVA:CE nanofibers showed no toxicity against fibroblast cells. The cell culture results exhibited the proper biocompatibility and proliferation on both CE-loaded and unloaded webs. The antibacterial test showed that CE-loaded nanofibrous web is capable of improving the antibacterial activity about 99% and 97% against gram-positive and gram-negative bacteria, respectively. Therefore, the CE-loaded web can be used as an antimicrobial wound dressing for healing improvement.

摘要

在本研究中，海藻酸钠 (SA): 聚乙烯醇 (PVA) 与不同浓度的豆蔻提取物 (CE) 共混物被静电纺丝。SEM 图像显示 CE 的最佳含量为 10 wt%，导致平均直径为 233 ± 33 nm 的纤维。FTIR 光谱用于研究聚合物和 CE 之间可能的相互作用。CE 释放遵循一级动力学，在所需抗菌活性所需的前 24 小时内有 86% 的初始突释。为了研究生物学特性，进行了MTT测定、细胞粘附和抗菌试验。SA:PVA:CE 纳米纤维对成纤维细胞没有毒性。细胞培养结果在 CE 加载和未加载的网上都表现出适当的生物相容性和增殖。抗菌试验表明，载有 CE 的纳米纤维网对革兰氏阳性菌和革兰氏阴性菌的抗菌活性分别提高了 99% 和 97%。因此，加载 CE 的网可用作抗菌伤口敷料以改善愈合。