Specific PCR‐based Detection of Phomopsis heveicola the Cause of Leaf Blight of Coffee ( Coffea arabica L.) in China,Letters in Applied Microbiology

当前位置： X-MOL 学术 › Lett. Appl. Microbiol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Specific PCR‐based Detection of Phomopsis heveicola the Cause of Leaf Blight of Coffee ( Coffea arabica L.) in China
Letters in Applied Microbiology ( IF 2.0 ) Pub Date : 2020-12-18 , DOI: 10.1111/lam.13396
Y Lu ₁ , W H Wu ₁ , C P He ₁ , Y Q Liang ₁ , X Huang ₁ , J L Zheng ₁ , J G Xi ₁ , S B Tang ₁ , K X Yi ₁

Affiliation

Coffee (Coffea arabica L.), is currently grown in many tropical and subtropical areas countries and is a major traded commodity for the developing world. Coffee leaf blight, caused by Phomopsis heveicola, is one of the most important fungal diseases dangerous to coffee crops in China. This study aimed to develop a PCR-based diagnostic method for detecting Phomopsis heveicola in planta. Specific primers (CPHF /CPHR) were designed based on sequence data of region of internal transcribed spacer (ITS1 and ITS4) of P. heveicola. The efficiency and specificity of CPHF/CPHR were established by PCR analysis of DNA from P. heveicola strains isolated from China and fungal isolates of other genera. A single amplification product of 318 bp was detected from DNA P. heveicola isolates. No amplification product was observed with any of the other fungal isolates tested. The specific primers designed and employed in PCR detected as low as 3 pg of P. heveicola DNA. This is the first report on the development of a species-specific PCR assay for identification and detection of P. heveicola. Thus, the PCR-based assay developed was very specific, rapid, and sensitive tool for the detection of pathogen P. heveicola..

更新日期：2020-12-18

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11