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A reassessment of the diversity of green toads (Bufotes) in the circum‐Aegean region
Integrative Zoology ( IF 3.5 ) Pub Date : 2020-09-25 , DOI: 10.1111/1749-4877.12494
Christophe DUFRESNES 1 , Nikolas M. PROBONAS 2 , Ilias STRACHINIS 3
Affiliation  

The dynamic biogeography of glacial refugia may cause complex patterns of genetic admixture between parapatric taxa, which in turn can mislead their systematics, diversity, and distributions. We investigated this issue for green toads (Bufotes) inhabiting the circum‐Aegean region, a biodiversity hotspot of the Eastern Mediterranean. A previous phylogeographic study based on mitochondrial and microsatellite loci identified the hybrid zone between the European (viridis) and Anatolian (sitibundus) lineages of B. viridis all over the Balkan Peninsula, but subsequent range‐wide genomic analyses (>1000 SNPs) located this transition in Turkey, a thousand kilometers eastwards. In order to clarify the diversity and taxonomy of the circum‐Aegean populations, we reconciled these conflicting findings by integrating previous data with pure sitibundus individuals. Our results confirmed that the viridis/sitibundus hybrid zone extends in Western Anatolia, but that southeastern European populations feature cytonuclear discordances and a high and structured microsatellite diversity. This remarkable situation may stem from a massive geographic displacement of the hybrid zone during the last glacial fluctuations, an underappreciated yet seemingly common feature among the herpetofauna of the region. Our study thus contributes to the rising view that mitochondrial DNA can be a poor predictor of current phylogeographic structure, hence the need for genomic data, especially for narrowly distributed taxa. Finally, the analyses unambiguously support the distinction of a micro‐endemic clade of green toads unique to some Cyclades islands, for which we provide a formal taxonomic description.

中文翻译:

重新评估爱琴海地区的绿色蟾蜍(Bufotes)的多样性

冰川避难所的动态生物地理学可能会导致准家族类群之间遗传混合的复杂模式,进而可能误导其系统性,多样性和分布。我们调查了绿蟾蜍(这个问题Bufotes)居住在circum -爱琴海地区,地中海的生物多样性热点地区。根据查明的欧洲(之间的混合区线粒体和微卫星位点先前的系统地理学研究贻贝)和安那托利亚(sitibundus)的谱系B.贻贝在整个巴尔干半岛上,但随后的范围广泛的基因组分析(> 1000个SNP)位于土耳其的这一过渡区,向东1000公里。为了阐明环爱琴海种群的多样性和分类学,我们通过将以前的数据与纯西伯来书个体进行整合来调和这些矛盾的发现。我们的研究结果证实了绿//西氏und鱼杂种带延伸到安纳托利亚西部,但东南欧人口具有细胞核不一致和高度结构化的微卫星多样性的特征。这种显着情况可能是由于上一次冰川波动期间混合带发生了巨大的地理位移,这是该地区的爬山虎属动物之间未被充分认识到的,但看似普遍的特征。因此,我们的研究促使人们越来越多地认为线粒体DNA不能很好地预测当前的系统地理结构,因此需要基因组数据,特别是对于分布较窄的分类单元。最后,分析明确地支持了一些基克拉迪群岛特有的绿色蟾蜍微地方性进化支的区分,为此我们提供了正式的分类学描述。
更新日期:2020-09-25
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