New evidence suggests that glycan expression in placental cells of women with invasive disorders of pregnancy differs from that in normal pregnant women. Hypothesizing that modifications of glycan expression could account for the course of preeclampsia, we established placental villous histocultures and compared glycan expression in women with preeclampsia with that in normal pregnant women and also in syncytialized BeWo cells, and we tested the effect of glycan expression on the functional phenotypes of circulating natural killer (NK) cells. Histocultures of third-trimester placentae from women with preeclampsia and full-term placentae from healthy pregnant women and BeWo choriocarcinoma cells were assessed for the expression of terminal glycans by lectin-binding assays. Circulating NK cells from nonpregnant healthy donors were tested in vitro for their cytotoxic activity and intracellular cytokine content. Histocultures from women with preeclampsia expressed significantly more mannose than did those from healthy pregnant women. Both histocultures and BeWo cells expressed terminal fucose, mannose, sialic acid, and N -acetylgalactosamine, although mean fluorescence intensity (MFI) expression was lower in choriocarcinoma cells than in cells from histocultures. Cocultures of circulating NK cells with K562 target cells resulted in a dose-dependent cytotoxicity effect, but the use of BeWo cells as target reduced cytotoxic activity; this reduction was not affected by syncytialization. Histocultures of placental villous tissue of women with preeclampsia expressed high levels of terminal mannose. We proposethat placental glycans may modulate the functional activity of circulating NK cells in the context of systemic inflammatory response in preeclampsia.

新的证据表明，患有侵袭性妊娠疾病的女性胎盘细胞中的聚糖表达与正常孕妇不同。假设聚糖表达的改变可以解释先兆子痫的过程，我们建立了胎盘绒毛组织培养物，并将先兆子痫女性的聚糖表达与正常孕妇以及合胞化 BeWo 细胞中的聚糖表达进行了比较，我们测试了聚糖表达对子痫前期的影响循环自然杀伤 (NK) 细胞的功能表型。通过凝集素结合测定评估来自先兆子痫妇女的妊娠晚期胎盘和来自健康孕妇的足月胎盘和 BeWo 绒毛膜癌细胞的组织培养物中末端聚糖的表达。测试了来自非怀孕健康供体的循环 NK 细胞体外细胞毒活性和细胞内细胞因子含量。先兆子痫妇女的组织培养物表达的甘露糖明显高于健康孕妇的组织培养物。组织培养物和 BeWo 细胞均表达末端岩藻糖、甘露糖、唾液酸和N-乙酰半乳糖胺，尽管绒毛膜癌细胞中的平均荧光强度 (MFI) 表达低于来自组织培养的细胞。循环 NK 细胞与 K562 靶细胞的共培养导致剂量依赖性细胞毒性作用，但使用 BeWo 细胞作为靶点会降低细胞毒性活性；这种减少不受合胞化的影响。先兆子痫妇女胎盘绒毛组织的组织培养表达高水平的终末甘露糖。我们提出，在先兆子痫的全身炎症反应的背景下，胎盘聚糖可能调节循环 NK 细胞的功能活性。