Abstract The present study investigates the purification and characterization of mosquito larvicidal lectin from the seed kernel extract of Annona muricata and toxic effects on non-target organism Chironomus costatus. Soursop lectin was purified by anion-exchange column chromatography using DEAE-cellulose with approximately molecular mass of 260 kDa and with seven distinct subunits (16, 18, 21, 22, 24, 73 and 95 kDa). Soursop lectin highest Hemagglutination (HA) titer value of 128 was recorded against hen indicator RBC type with the influence of different divalent cations such as Ca2+, Ba2+ and Mn2+. The lectin mediated HA activity was highly inhibited by monosaccharides of glucose and mannose and disaccharides such as trehalose and melibiose. It was found to be EDTA sensitive (at 30 mM), pH-dependent (between 6–9) and heat-labile (upto 60 °C). A significant homology for soursop lectin was recorded to leguminous seed lectin from Psophocarpus scandens in MALDI-TOF-MS analysis with 66 % of sequence coverage. Finally, the toxicity bioassay of soursop lectin resulted in 100 % larval mortality for A. aegypti at 48 h whereas only 10 % mortality recorded to non-target organism C. costatus. Therefore, we concluded that soursop lectin could be a potent insecticidal agent in integrated pest management for controlling various insect pests.

中文翻译：

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番荔枝杀蚊子凝集素的纯化、表征及其对非靶标生物的生态毒性作用

摘要 本研究研究了番荔枝籽仁提取物中杀蚊幼虫凝集素的纯化和表征及其对非靶标生物摇蚊的毒性作用。Soursop 凝集素通过阴离子交换柱色谱法纯化，使用 DEAE 纤维素，分子量约为 260 kDa，具有七个不同的亚基（16、18、21、22、24、73 和 95 kDa）。在不同二价阳离子如 Ca2+、Ba2+ 和 Mn2+ 的影响下，针对母鸡指示剂 RBC 类型记录了 Soursop 凝集素最高血凝 (HA) 滴度值 128。凝集素介导的HA活性被葡萄糖和甘露糖的单糖以及海藻糖和蜜二糖等二糖高度抑制。发现它对 EDTA 敏感（30 mM）、pH 依赖性（6-9 之间）和热不稳定（高达 60°C）。在 MALDI-TOF-MS 分析中，刺果番荔枝凝集素与来自 Psophocarpus scandens 的豆科植物种子凝集素具有显着的同源性，序列覆盖率为 66%。最后， soursop 凝集素的毒性生物测定导致 A. aegypti 在 48 小时内的幼虫死亡率为 100%，而非靶标生物 C.costatus 的死亡率仅为 10%。因此，我们得出结论，刺果番荔枝凝集素可以作为一种有效的杀虫剂，用于防治各种害虫的综合害虫管理。