Point-of-care risk assessment (PCRA) for airborne viruses requires a system that can enrich low-concentration airborne viruses dispersed in field environments into a small volume of liquid. In this study, airborne virus particles were collected to a degree above the limit of detection (LOD) for a real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). This study employed an electrostatic air sampler to capture aerosolized test viruses (human coronavirus 229E (HCoV-229E), influenza A virus subtype H1N1 (A/H1N1), and influenza A virus subtype H3N2 (A/H3N2)) in a continuously flowing liquid (aerosol-to-hydrosol (ATH) enrichment) and a concanavalin A (ConA)-coated magnetic particles (CMPs)-installed fluidic channel for simultaneous hydrosol-to-hydrosol (HTH) enrichment. The air sampler's ATH enrichment capacity (EC) was evaluated using the aerosol counting method. In contrast, the HTH EC for the ATH-collected sample was evaluated using transmission-electron-microscopy (TEM)-based image analysis and real-time qRT-PCR assay. For example, the ATH EC for HCoV-229E was up to 67,000, resulting in a viral concentration of 0.08 PFU/mL (in a liquid sample) for a viral epidemic scenario of 1.2 PFU/m³ (in air). The real-time qRT-PCR assay result for this liquid sample was “non-detectable” however, subsequent HTH enrichment for 10 min caused the “non-detectable” sample to become “detectable” (cycle threshold (CT) value of 33.8 ± 0.06).

空气传播病毒的即时风险评估 (PCRA) 需要一个能够将分散在现场环境中的低浓度空气传播病毒富集到少量液体中的系统。在这项研究中，空气传播的病毒颗粒被收集到高于检测限 (LOD) 的程度，用于实时定量逆转录聚合酶链式反应 (qRT-PCR)。本研究采用静电空气采样器捕获连续流动液体中的雾化测试病毒（人类冠状病毒 229E (HCoV-229E)、甲型流感病毒 H1N1 亚型 (A/H1N1) 和甲型流感病毒 H3N2 亚型 (A/H3N2)） （气溶胶到水溶胶 (ATH) 富集）和安装有刀豆球蛋白 A (ConA) 涂层磁性颗粒 (CMP) 的流体通道，用于同时进行水溶胶到水溶胶 (HTH) 富集。使用气溶胶计数方法评估空气采样器的ATH富集能力（EC）。相比之下，使用基于透射电子显微镜 (TEM) 的图像分析和实时 qRT-PCR 测定来评估 ATH 收集的样品的 HTH EC。例如，HCoV-229E 的 ATH EC 高达 67,000，导致病毒浓度为 0.08 PFU/mL（液体样品中），而病毒流行情况为 1.2 PFU/m ³（空气中）。该液体样品的实时 qRT-PCR 检测结果为“不可检测”，然而，随后 10 分钟的 HTH 富集导致“不可检测”样品变为“可检测”（循环阈值 (CT) 值为 33.8 ± 0.06）。