Abstract

Adult granary weevils feeding on grain responded selectively to the volatile compounds of different strains of the entomopathogenic fungi Beauveria bassiana and Lecanicillium muscarium. The mycelium of B. bassiana strains had a repellent effect or a neutral effect with a tendency toward repellency. The response to L. muscarium strains varied from weak repellent (G-033) and neutral (Vl 49) to attractive one (Vl 21). Beauveria bassiana strain Bb 17 showed the highest virulence after beetles were sprayed with conidial suspension containing 1.0 × 10⁸ spores / ml: the mortality of the beetles on the 7th day reached 74% with extensive mycelial growth over the dead beetles, LT₅₀ was 5.6 days for this strain and 23 days for strain Bb 13. The L. muscarium strains showed low virulence, with the exception of strain G-033 (LT₅₀ 15.3 days), and they also formed mycelium on the dead insects. The granary weevil response should be considered when assessing the potential of entomopathogenic fungi. Beauveria bassiana strain Bb 17 was selected as a promising pest control agent, since neutral reaction of the granary weevil to its mycelium facilitates the spreading of fungal infection.

中文翻译：

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致病性真菌球孢白僵菌（Bals.-Criv。）Vuill的挥发性化合物的影响。和Lecanicillium muscarium R. Zare等人（W. Gams）对Sitophilus granarius（L。）（鞘翅目，Dryophthoridae）行为的评估以及这些真菌不同菌株的毒力评估

摘要

以谷物为食的成年粮象鼻虫对不同病原真菌球孢白僵菌和蕈状Lecanicillium muscarium的挥发性化合物有选择性的反应。球孢杆菌菌株的菌丝体具有驱避作用或中性作用，具有驱避作用的趋势。对穆氏镰刀菌菌株的响应从弱驱蚊剂（G-033）和中性驱虫剂（Vl 49）到诱人的（Vl 21）不等。球孢白僵菌Bb 17菌株在甲虫喷洒含1.0×10 ⁸孢子/ ml的分生孢子悬浮液后显示出最高的毒力：在第7天，甲虫的死亡率达到74％，菌丝在死甲虫LT上广泛繁殖该菌株的₅₀天为5.6天，而Bb 13菌株为23天。除G-033菌株（LT ₅₀ 15.3天）外，粘液梭状芽孢杆菌菌株显示出低毒力，它们还在死虫上形成菌丝体。在评估昆虫病原性真菌的潜力时，应考虑粮仓象鼻虫的反应。球孢白僵菌菌株Bb 17被选为有前途的害虫防治剂，因为粮象鼻虫对其菌丝体的中性反应可促进真菌感染的传播。