Globally, viral diseases cause huge economic losses in crops and their management is a big challenge to growers as well as researchers. Mixed infection is the existence of more than one virus in single plant, which results in varied symptoms at the same time. The presence of more than one virus always leads to difficulty in understanding the etiology of disease. Most of the viral diseases go unnoticed either due to the latent nature of infection of virus(es) or due to low severity of symptoms. But this might be true in case of single infection of the host by the concerned virus. When such viruses are seen causing infection in combination with other viruses at particular time, more severe disease symptoms can be observed. For any successful management of viral disease especially during mixed infection, detection and identification of plant viruses causative of the disease are of foremost importance. Several approaches like cocktail ELISA, multiplex PCR for known viruses and next-generation sequencing for both known and unknown viruses have been developed for detection of mixed infection of viruses. During mixed infection, several kinds of interaction commonly referred to as synergistic or antagonistic interactions are going on between and among the viruses, which aggravate the disease with more severe symptoms than with single infections. Here, we review the mixed infection of viruses, methods of detection, factors influencing, interactions and impact on plant during mixed infection.

在全球范围内，病毒性疾病会给农作物造成巨大的经济损失，其管理对种植者和研究人员都是巨大的挑战。混合感染是指单个植物中存在多种病毒，这会同时导致多种症状。不止一种病毒的存在总是导致难以理解疾病的病因。由于病毒感染的潜在性质或症状严重程度较低，大多数病毒性疾病未引起注意。但这在有关病毒对宿主进行一次单一感染的情况下可能是正确的。当看到此类病毒在特定时间与其他病毒一起引起感染时，可以观察到更严重的疾病症状。对于病毒性疾病的任何成功管理，尤其是在混合感染期间，最重要的是检测和鉴定引起该病的植物病毒。已经开发了几种方法，例如鸡尾酒ELISA，已知病毒的多重PCR和已知和未知病毒的下一代测序，以检测病毒的混合感染。在混合感染期间，病毒之间以及病毒之间正在发生几种通常称为协同或拮抗作用的相互作用，这使该疾病的症状比单一感染更为严重。在这里，我们回顾了混合感染期间病毒的混合感染，检测方法，影响因素，相互作用和对植物的影响。已经开发出用于已知病毒的多重PCR和用于已知和未知病毒的下一代测序，以检测病毒的混合感染。在混合感染期间，病毒之间以及病毒之间正在发生几种通常称为协同或拮抗作用的相互作用，这使该疾病的症状比单一感染更为严重。在这里，我们回顾了混合感染期间病毒的混合感染，检测方法，影响因素，相互作用和对植物的影响。已经开发出用于已知病毒的多重PCR和用于已知和未知病毒的下一代测序，以检测病毒的混合感染。在混合感染期间，病毒之间以及病毒之间正在发生几种通常称为协同或拮抗作用的相互作用，这使该疾病的症状比单一感染更为严重。在这里，我们回顾了混合感染期间病毒的混合感染，检测方法，影响因素，相互作用和对植物的影响。与单一感染相比，这种疾病加重了更严重的症状。在这里，我们回顾了混合感染期间病毒的混合感染，检测方法，影响因素，相互作用和对植物的影响。与单一感染相比，这种疾病加重了更严重的症状。在这里，我们回顾了混合感染期间病毒的混合感染，检测方法，影响因素，相互作用和对植物的影响。