Background

The acute hypoxic injury caused by the plain population entering the plateau in a short period of time has become the main cause of endangering the health of the people who rush into the plateau.

Objective

The study aimed to identify the key genes which participate in resisting the acute hypoxic injury in SD Rats by transcriptomic profile analysis.

Methods

48 Sprague Dawley (SD) male rats were enrolled and randomly divided into four groups (0h, 24h, 48h, 72h) and housed in hypobaric hypoxia chamber with altitude 6000m for different periods of time to make them acute hypoxic injury. The transcriptomic profile of the lung tissue of the rats was analysed by RNA second-generation sequencing combined with bioinformatics analysis.

Results

The results of GO and KEGG function classification analysis revealed that the differential expression genes enriched in steroid hormone synthesis pathway especially in 48h group compared to F0 group. Further analysis revealed that Farnesyl Diphosphate Farnesyl Transferase 1 (fdft1) gene encoding a rate-limiting enzyme in steroid hormone synthesis pathway was significant differently expressed between the groups. The expression levels of fdft1 gene were further verified by RT-PCR and Western-blot methods.

Conclusions

The results suggest that fdft1 gene plays an important role in responding to acute hypoxic injury by regulating steroid hormone biosynthesis.

中文翻译：

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fdft1在类固醇激素合成通路中对SD大鼠急性缺氧损伤的保护机制

背景

平原人群短时间内进入高原造成的急性缺氧性损伤，已成为危害冲入高原人群健康的主要原因。

客观的

本研究旨在通过转录组学分析确定参与抵抗SD大鼠急性缺氧损伤的关键基因。

方法

48只Sprague Dawley(SD)雄性大鼠随机分为4组(0h、24h、48h、72h)，分别饲养于海拔6000m的低压缺氧室中，不同时间进行急性缺氧损伤。通过RNA二代测序结合生物信息学分析来分析大鼠肺组织的转录组学特征。

结果

GO和KEGG功能分类分析结果显示，与F0组相比，48h组类固醇激素合成途径中差异表达基因富集。进一步分析表明，在类固醇激素合成途径中编码限速酶的法呢基二磷酸法呢基转移酶1( fdft1 )基因在各组间的表达存在显着差异。通过RT-PCR和Western-blot方法进一步验证了fdft1基因的表达水平。

结论

结果表明fdft1基因通过调节类固醇激素的生物合成在应对急性缺氧损伤中发挥重要作用。