The nematode Caenorhabditis elegans (C. elegans) is a convenient tool to evaluate iron metabolism as it shares great orthology with human proteins involved in iron transport, in addition to being transparent and readily available. In this work, we describe how wild-type (N2) C. elegans nematodes in the first larval stage can be loaded with acetomethoxycalcein (CAL-AM) and study it as a whole-organism model for both iron speciation and chelator permeability of the labile iron pool (LIP). This model may be relevant for high throughput assessment of molecules intended for chelation therapy of iron overload diseases.

中文翻译：

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铁过载秀丽隐杆线虫中铁运输和螯合的高通量荧光评估

线虫秀丽隐杆线虫( C. elegans ) 是一种评估铁代谢的便捷工具，因为它与参与铁转运的人类蛋白质有着很大的同源性，而且透明且易于获得。在这项工作中，我们将描述如何野生型（N2）秀丽隐杆线虫中的第一幼虫阶段的线虫可以与acetomethoxycalcein（CAL-AM）加载并研究它作为既铁形态和螯合剂渗透性的一个全生物体模型不稳定铁池（LIP）。该模型可能与用于铁过载疾病螯合治疗的分子的高通量评估有关。