In the current study, aerial parts (leaves, stem and shoots) of C. album were extracted with methanol and subjected to phytochemical and HPLC analysis. Agar well diffusion method was used for anti-bacterial activity against Gram-negative strains Escherichia coli, Salmonella typhi, Klebsiella, Pseudomonas aeruginosa and Gram-positive Bacillus cereus, Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus. Burn was induced through flame heated metal rod on mice. C. album ointment (2% w/w), Vaseline (vehicle) and silver sulfadiazine (standard) were topically applied thrice daily for 15 days. Wound area was measured on day 0, 5, 10 and 15. On last day, the wound tissues were excised and subjected to histopathological, quantitative PCR and immunohistochemical analysis. Phenols, alkaloids, phytosterols, tannins, saponins, flavonoids, carbohydrates and glycosides were detected in phytochemical analysis. HPLC chromatogram displayed peaks for rutin, quercetin, ascorbic acid, gallic acid and various other phyto-constituents. The extract exhibited zone of inhibition in millimeter (mm) against E. coli (12.3 ± 0.57), S. typhyi (14.6 ± 1.52), Klebsiella (11.8 ± 0.76), P. aeruginosa (12.3 ± 0.57), B. cereus (12.5 ± 1.29), S. aureus (18.3 ± 2.08), and MRSA (11.8 ± 0.76). The wound area in C. album group was significantly (60%) reduced as compared to vehicle group (11%). Histological analysis showed complete re-epithelialization and fine tissue in extract treated group. qPCR data revealed up-regulation of EGF, PDGF and TGF-β1 genes in extract treated group. Similarly, immunohistochemistry results confirmed heightened EGFR expression in extract treated group. Our findings suggest that C. album can promote wound healing and tissue regeneration through control of burns related infection and modulation of growth factors and its receptors.

在目前的研究中，地上部分（叶，茎和芽）的藜用甲醇萃取，并进行植物化学和HPLC分析。琼脂孔扩散法用于对革兰氏阴性菌株大肠杆菌、伤寒沙门氏菌、克雷伯氏菌、铜绿假单胞菌和革兰氏阳性蜡状芽孢杆菌、金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌具有抗菌活性。通过火焰加热金属棒在小鼠身上引起烧伤。C.专辑软膏（2% w/w）、凡士林（载体）和磺胺嘧啶银（标准）每天局部应用三次，共 15 天。在第0、5、10和15天测量伤口面积。最后一天，切除伤口组织并进行组织病理学、定量PCR和免疫组织化学分析。在植物化学分析中检测到酚类、生物碱、植物甾醇、单宁、皂苷、黄酮类、碳水化合物和糖苷。HPLC 色谱图显示芦丁、槲皮素、抗坏血酸、没食子酸和各种其他植物成分的峰。提取物对大肠杆菌(12.3 ± 0.57)、S表现出以毫米 (mm) 为单位的抑菌圈。伤寒杆菌(14.6 ± 1.52)、克雷伯氏菌(11.8 ± 0.76)、铜绿假单胞菌(12.3 ± 0.57) 、蜡样芽孢杆菌(12.5 ± 1.29)、金黄色葡萄球菌(18.3 ± 2.08) 和 MRSA (11.8 ± 0.76)。与载体组 (11%) 相比，C. album组的伤口面积显着 (60%) 减少。组织学分析显示提取物处理组完全再上皮化和精细组织。qPCR 数据显示提取物处理组中EGF、PDGF和TGF-β1基因的上调。类似地，免疫组织化学结果证实提取物治疗组中EGFR表达升高。我们的研究结果表明，C. album可以通过控制烧伤相关感染和调节生长因子及其受体来促进伤口愈合和组织再生。