S-100B is used in melanoma follow-up. This serum biomarker is also present in adipocytes; therefore, subcutaneous adipocytes trapped in the needle before performing a venipuncture could contaminate the serum. The aim was to study the influence of adipocyte contamination on blood samples used for S-100B analysis, possibly resulting in falsely elevated S-100B values. A total of 294 serum samples were collected from 147 American Joint Committee on Cancer staging stage III melanoma patients. The mean difference between the first (dummy) and second tubes was 0.003 μg/l (p = 0.077), with a decrease in the second tube. Compared with the second tube, the S-100B level was higher in the first tube in 33.3% of the samples, equal in 36.8% of the samples and lower in 29.9% of the samples. No significant difference between the two consecutively drawn tubes was found. There seems to be no necessity of implementing a dummy tube system for accurate S-100B determination in melanoma patients.

中文翻译：

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在黑色素瘤患者中进行充分的 S-100B 测定不需要双静脉穿刺。

S-100B 用于黑色素瘤随访。这种血清生物标志物也存在于脂肪细胞中。因此，在进行静脉穿刺之前被困在针中的皮下脂肪细胞可能会污染血清。目的是研究脂肪细胞污染对用于 S-100B 分析的血液样本的影响，这可能导致 S-100B 值虚假升高。从 147 名美国癌症联合委员会分期 III 期黑色素瘤患者中收集了总共 294 份血清样本。第一个（虚拟）和第二个试管之间的平均差异为 0.003 μg/l (p = 0.077)，第二个试管中的差异较小。与第二管相比，第一管中 S-100B 水平在 33.3% 的样品中较高，在 36.8% 的样品中相等，在 29.9% 的样品中较低。未发现两个连续拉制的管之间存在显着差异。似乎没有必要实施一个假管系统来准确测定黑色素瘤患者的 S-100B。