Background:SARS-CoV-2 infection is associated with myocardial injury, but there is a paucity of experimental platforms for the condition.

Methods and Results:Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) infected by SARS-CoV-2 for 3 days ceased beating and exhibited cytopathogenic changes with reduced viability. Active viral replication was evidenced by an increase in supernatant SARS-CoV-2 and the presence of SARS-CoV-2 nucleocaspid protein within hiPSC-CMs. Expressions of BNP, CXCL1, CXCL2, IL-6, IL-8 and TNF-α were upregulated, while ACE2 was downregulated.

Conclusions:Our hiPSC-CM-based in-vitro SARS-CoV-2 myocarditis model recapitulated the cytopathogenic effects and cytokine/chemokine response. It could be exploited as a drug screening platform.

背景： SARS-CoV-2感染与心肌损伤有关，但该病缺乏实验平台。

方法和结果： SARS-CoV-2感染人类诱导的多能干细胞衍生的心肌细胞（hiPSC-CMs）持续3天，并停止跳动，并表现出细胞致病性变化，活力降低。通过上清液SARS-CoV-2的增加和hiPSC-CM中SARS-CoV-2核壳蛋白的存在来证明病毒活跃复制。BNP，CXCL1，CXCL2，IL-6，IL-8和TNF-α的表达上调，而ACE2的表达下调。

结论：我们基于hiPSC-CM的体外SARS-CoV-2心肌炎模型概括了细胞致病作用和细胞因子/趋化因子反应。它可以用作药物筛选平台。