Introduction: Apheresis platelets (APs) are clinically and crucially important in the prevention and treatment of bleeding in patients with thrombocytopenia or cancer. However, few researchers have addressed the variation of supernatant metabolites and exosome proteins in APs during storage and their effects on cancer cell proliferation. Objective: This study was designed to explore the change rules of the metabolites and exosomal proteins of APs during storage and their effects on cancer cell proliferation. Methods: Metabolomics and proteomics were separately applied to analyze the variation of AP supernatant metabolites and exosomal proteins between freshly prepared day-0 and day-5 terminal-stored APs. Cell counting kit (CCK)-8 assay was performed to detect the effects of AP supernatants and exosomes on the proliferation of cancer cells. Results: We found that the supernatant metabolites and exosomal proteins in APs were significantly different on day 0 and day 5, and that many differential metabolites and exosomal proteins were associated with cancer characteristics. Furthermore, the day-5 AP supernatants had a greater inhibition of the proliferation of K562, HepG2, and HCT116 cancer cells, but the day-5 AP exosomes had no significant effect on the proliferation of these cancer cells. Conclusion: The variant terminal-stored AP supernatants may inhibit the proliferation of cancer cells but the variant terminal AP exosomes have no effect on cancer cell proliferation.
Transfus Med Hemother

中文翻译：

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代谢组学和蛋白质组学揭示单采血小板储存过程中物质的变化及其对癌细胞增殖的影响

简介：单采血小板 (AP) 在临床上对于预防和治疗血小板减少症或癌症患者的出血至关重要。然而，很少有研究人员解决APs在储存过程中上清代谢物和外泌体蛋白的变化及其对癌细胞增殖的影响。目的：本研究旨在探讨APs在储存过程中代谢物和外泌体蛋白的变化规律及其对癌细胞增殖的影响。方法：分别应用代谢组学和蛋白质组学分析新鲜制备的第0天和第5天终端储存的AP之间AP上清代谢物和外泌体蛋白的变化。采用细胞计数试剂盒（CCK）-8检测AP上清液和外泌体对癌细胞增殖的影响。结果：我们发现AP中的上清代谢物和外泌体蛋白在第0天和第5天显着不同，并且许多差异代谢物和外泌体蛋白与癌症特征相关。此外，第5天的AP上清液对K562、HepG2和HCT116癌细胞的增殖有更大的抑制作用，但第5天的AP外泌体对这些癌细胞的增殖没有显着影响。结论：变异末端AP上清液可能抑制癌细胞增殖，但变异末端AP外泌体对癌细胞增殖没有影响。
输血医学母乳