Current plant-derived anticancer therapeutics aim to reach higher effectiveness, to potentiate chemosensitivity and minimize the toxic side effects compared to conventional chemotherapy. Cotinus coggygria Scop. is a herb with high pharmacological potential, widely applied in traditional phytotherapy. Our previous study revealed that leaf aqueous ethanolic extract from C. coggygria exerts in vitro anticancer activity on human breast, ovarian and cervical cancer cell lines. The objective of the present research was to investigate possible molecular mechanisms and targets of the antitumor activity of the extract in breast cancer MCF7 cells through analysis of cell cycle and apoptosis, clonogenic ability assessment, evaluation of the extract genotoxic capacity, characterization of cells thermodynamic properties, and analysis on the expression of genes involved in cellular epigenetic processes. The obtained results indicated that in MCF7 cells C. coggygria extract causes S phase cell cycle arrest and triggers apoptosis, reduces colony formation, induces DNA damage, affects cellular thermodynamic parameters, and tends to inhibit the relative expression of DNMT1, DNMT3a, MBD3, and p300. Further studies on the targeted molecules and the extract anti-breast cancer potential on animal experimental model system, need to be performed in the future.

中文翻译：

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Cotinus coggygria Scop。在 MCF7 乳腺癌细胞中诱导细胞周期停滞、细胞凋亡、基因毒性作用、热力学和表观遗传事件

与传统化学疗法相比，目前的植物衍生抗癌疗法旨在达到更高的有效性，增强化学敏感性并最大限度地减少毒副作用。Cotinus coggygria Scop。是一种具有很高药理潜力的草药，广泛应用于传统的植物疗法。我们之前的研究表明，来自 C. coggygria 的叶水乙醇提取物对人乳腺癌、卵巢癌和宫颈癌细胞系具有体外抗癌活性。本研究的目的是通过细胞周期和细胞凋亡分析、克隆能力评估、提取物遗传毒性能力评估、细胞热力学特性表征，研究提取物在乳腺癌 MCF7 细胞中抗肿瘤活性的可能分子机制和靶点。 , 和分析参与细胞表观遗传过程的基因的表达。所得结果表明，在MCF7细胞中，C. coggygria提取物引起S期细胞周期停滞并触发细胞凋亡，减少集落形成，诱导DNA损伤，影响细胞热力学参数，并倾向于抑制DNMT1、DNMT3a、MBD3和p300。未来需要在动物实验模型系统上进一步研究靶向分子和提取物抗乳腺癌的潜力。DNMT3a、MBD3 和 p300。未来需要在动物实验模型系统上进一步研究靶向分子和提取物抗乳腺癌的潜力。DNMT3a、MBD3 和 p300。未来需要在动物实验模型系统上进一步研究靶向分子和提取物抗乳腺癌的潜力。