Abstract

Bone marrow mesenchymal stem cells (BMSCs), as seed cells, have played an important role in bone defect repair. However, efficiently amplifying and inducing BMSCs in vitro or vivo remains an urgent problem to be solved. Electrical stimulation has been beneficial to the proliferation and differentiation of BMSCs, but current electrical stimulation methods have a critical disadvantage in that they usually burn the skin. g-C₃N₄/rGO, a new photosensitive material, can produce photocurrent under natural light irradiation, thus reducing energy consumption. Our purpose was to explore whether this photocurrent can promote the proliferation and differentiation of BMSCs. g-C₃N₄/rGO synthesised under high temperature and pressure had negligible cytotoxicity as confirmed by methyl thiazolyl tetrazolium to BMSCs. Better osteogenesis was found in the blue light material group than in the light-shielding material group, exhibited by alizarin red staining, alkaline phosphatase activity, Western-Blot, and RT-qPCR. Animal experiments showed that the bone repair potential of the material group was significantly higher than that of the non-material group. Overall, we conclude that g-C₃N₄/rGO is a new non-toxic photosensitive material which can rapidly induce BMSCs into osteoblasts, accelerating bone regeneration and providing us with a feasible method of rapid bone repair.

摘要

骨髓间充质干细胞（BMSCs）作为种子细胞，在骨缺损修复中发挥了重要作用。然而，在体外或体内有效地扩增和诱导 BMSCs仍然是一个亟待解决的问题。电刺激有利于 BMSCs 的增殖和分化，但目前的电刺激方法有一个严重的缺点，它们通常会灼伤皮肤。gC ₃ N ₄ /rGO是一种新型光敏材料，可在自然光照射下产生光电流，从而降低能耗。我们的目的是探索这种光电流是否可以促进 BMSCs 的增殖和分化。气相色谱₃ N ₄甲基噻唑基四唑证明在高温高压下合成的 /rGO 对 BMSC 的细胞毒性可以忽略不计。通过茜素红染色、碱性磷酸酶活性、蛋白质印迹和 RT-qPCR 显示，蓝光材料组比遮光材料组具有更好的成骨作用。动物实验表明，材料组的骨修复潜力明显高于非材料组。总的来说，我们得出结论，gC ₃ N ₄ /rGO 是一种新型的无毒光敏材料，可以快速诱导 BMSCs 成为成骨细胞，加速骨再生，为我们提供了一种可行的快速骨修复方法。