Abstract

In the prevention of epidemic and pandemic emerging and neglected viral infections, natural products are an important source of lead compounds. Hornstedtia bella Škorničkis is a rhizomatous herb growing in the forest of central Vietnam. Hornstedtia bella essential oil (Hb EO) was recently characterised by our group as endowed of antimicrobial activity against Staphylococcus aureus Methicillin-Resistant strains. Here, we describe for the first time the evaluation of Hb EO against a spectrum of viruses responsible for important human diseases. Hb EO resulted active against Vaccinia virus (VV) (EC₅₀ values 80 μg/mL), closely related to variola virus, causative agent of smallpox. Hb EO was able to strongly reduce the viral VV titer in cell-based assay at not cytotoxic concentration and its potential mode of action was characterised by virucidal activity evaluation followed by time-of-addition assay. Furthermore, Hb EO antiviral activity was implemented in a combination study with the mycophenolic acid.

摘要

在预防流行和大流行新出现和被忽视的病毒感染中，天然产物是先导化合物的重要来源。Hornstedtia bella Škorničkis 是一种根茎草本植物，生长在越南中部的森林中。Hornstedtia Bella精油 (Hb EO) 最近被我们的小组描述为对金黄色葡萄球菌耐甲氧西林菌株具有抗菌活性。在这里，我们首次描述了 Hb EO 针对一系列导致重要人类疾病的病毒的评估。Hb EO 对痘苗病毒 (VV) (EC _{50 ) 具有活性}值 80 μg/mL)，与天花病原体天花病毒密切相关。在非细胞毒性浓度下，Hb EO 能够在基于细胞的测定中强烈降低病毒 VV 滴度，其潜在作用模式的特征在于杀病毒活性评估，然后是添加时间测定。此外，在与霉酚酸的联合研究中实施了 Hb EO 抗病毒活性。