当前位置: X-MOL 学术Biotechnol. J. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Multimodal Imaging and Soft X‐Ray Tomography of Fluorescent Nanodiamonds in Cancer Cells
Biotechnology Journal ( IF 3.2 ) Pub Date : 2020-09-24 , DOI: 10.1002/biot.202000289
Philipp Reineck 1 , Amanda N. Abraham 1 , Arpita Poddar 2 , Ravi Shukla 2 , Hiroshi Abe 3 , Takeshi Ohshima 3 , Brant C. Gibson 1 , Chaitali Dekiwadia 4 , José J. Conesa 5 , Eva Pereiro 5 , Amy Gelmi 6 , Gary Bryant 7
Affiliation  

Multimodal imaging promises to revolutionize the understanding of biological processes across scales in space and time by combining the strengths of multiple imaging techniques. Fluorescent nanodiamonds (FNDs) are biocompatible, chemically inert, provide high contrast in light‐ and electron‐based microscopy, and are versatile optical quantum sensors. Here it is demonstrated that FNDs also provide high absorption contrast in nanoscale 3D soft X‐ray tomograms with a resolution of 28 nm in all dimensions. Confocal fluorescence, atomic force, and scanning electron microscopy images of FNDs inside and on the surface of PC3 cancer cells with sub‐micrometer precision are correlated. FNDs are found inside ≈1 µm sized vesicles present in the cytoplasm, providing direct evidence of the active uptake of bare FNDs by cancer cells. Imaging artefacts are quantified and separated from changes in cell morphology caused by sample preparation. These results demonstrate the utility of FNDs in multimodal imaging, contribute to the understanding of the fate of FNDs in cells, and open up new possibilities for biological imaging and sensing across the nano‐ and microscale.

中文翻译:

癌细胞中荧光纳米金刚石的多峰成像和X射线软断层扫描

多峰成像有望通过结合多种成像技术的优势,彻底改变对跨时空尺度的生物过程的理解。荧光纳米金刚石(FND)具有生物相容性,化学惰性,在基于光和电子的显微镜中具有高对比度,并且是通用的光学量子传感器。在此证明,FND在纳米尺度的3D软X射线断层图中也可提供高吸收对比度,且在所有尺寸上的分辨率均为28 nm。PC3癌细胞内部和表面上FND的共聚焦荧光,原子力和扫描电子显微镜图像之间的亚微米精度是相关的。在细胞质中存在的大约1 µm大小的囊泡中发现了FND,这提供了癌细胞主动摄取裸FND的直接证据。量化成像伪像,并将其与由样品制备引起的细胞形态变化分开。这些结果证明了FND在多峰成像中的实用性,有助于理解细胞中FND的命运,并为跨纳米和微米级的生物成像和传感开辟了新的可能性。
更新日期:2020-09-24
down
wechat
bug