Label-free molecular detection of antibiotic susceptibility for Mycobacterium smegmatis using a low cost electrode format,Biotechnology and Applied Biochemistry

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Label-free molecular detection of antibiotic susceptibility for Mycobacterium smegmatis using a low cost electrode format
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-09-25 , DOI: 10.1002/bab.2037
Fatma Doğan Güzel ₁ , Hamed Ghorbanpoor ₁ , Araz Norouz Dizaji ₁ , Iremnur Akcakoca ₂ , Yasin Ozturk ₂ , Tanil Kocagoz _{3,

4} , Damion K Corrigan ₅ , Huseyin Avci ₆

Affiliation

Today, the emergence of antibiotic resistance in pathogenic bacteria is considered an important problem for society. Excessive consumption of antibiotics, long-term treatments, and inappropriate prescriptions continually increase the severity of the problem. Improving antibiotic stewardship requires improved diagnostic testing, and, therefore, in vitro antibiotic susceptibility testing is becoming increasingly important. This research details the development of an antibiotic susceptibility test for Mycobacterium smegmatis using streptomycin as antibiotics. This strain was selected because it is a member of the slow growing Mycobacterium genus and serves as a useful surrogate organism for M. tuberculosis. A commercially available and low-cost screen-printed gold electrode in combination with a specifically developed nucleic acid probe sequence for the 16SrRNA region of the mycobacterial genome was employed to monitor M. smegmatis nucleic acid sequences using the techniques of square-wave voltammetry and electrochemical impedance spectroscopy. The results show that it was possible to detect M. smegmatis sequences and distinguish antibiotic-treated cells from untreated cells with a label-free molecular detection. As a result, the in vitro antibiotic susceptibility test revealed that M. smegmatis showed sensitivity to streptomycin after a 24-H incubation, with the developed protocol representing a potential approach to determining antibiotic susceptibility more quickly and economically than current methods.

中文翻译：

使用低成本电极形式对耻垢分枝杆菌的抗生素敏感性进行无标记分子检测

今天，病原菌中抗生素耐药性的出现被认为是社会的一个重要问题。过量使用抗生素、长期治疗和不适当的处方不断增加问题的严重性。改进抗生素管理需要改进诊断测试，因此，体外抗生素敏感性测试变得越来越重要。这项研究详细介绍了使用链霉素作为抗生素对耻垢分枝杆菌进行抗生素敏感性试验的开发。选择该菌株是因为它是生长缓慢的分枝杆菌属的成员，并且可作为结核分枝杆菌的有用替代生物. 商业上可用的低成本丝网印刷金电极与专门开发的用于分枝杆菌基因组 16SrRNA 区域的核酸探针序列相结合，使用方波伏安法和电化学技术监测耻垢分枝杆菌核酸序列阻抗谱。结果表明，可以检测耻垢分枝杆菌序列，并通过无标记分子检测将抗生素处理的细胞与未处理的细胞区分开来。结果，体外抗生素敏感性试验表明，耻垢分枝杆菌在 24 小时孵育后显示出对链霉素的敏感性，所开发的方案代表了一种比现有方法更快、更经济地确定抗生素敏感性的潜在方法。

更新日期：2020-09-25

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