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The effects of exogenous cerium on photosystem II as probed by in vivo chlorophyll fluorescence and lipid production of Scenedesmus obliquus XJ002
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-09-24 , DOI: 10.1002/bab.2043 Jie Cheng 1, 2 , Xiongyan Du 3 , Huayang Long 4 , Han Zhang 1 , Xiang Ji 2
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-09-24 , DOI: 10.1002/bab.2043 Jie Cheng 1, 2 , Xiongyan Du 3 , Huayang Long 4 , Han Zhang 1 , Xiang Ji 2
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Cerium is the most abundant rare earth metal in the earth's crust, and it has deleterious effects on aquatic ecosystems from fertilizer runoff. Scenedesmus obliquus is an oil-rich microalga that grows rapidly and is sensitive to many kinds of toxins. Given that microalgae are useful indicators of eutrophication and toxic stress, it was found that lower concentrations of cerium (0.50–5.00 mg·L−1) stimulated algal growth and increased chlorophyll a content, whereas higher concentrations (above 50.00 mg·L−1) had an inhibitory effect on algal growth and chlorophyll a content. The algal growth rate and chlorophyll a content peaked at a cerium concentration of 5.00 mg·L−1. Both the donor and acceptor sides of photosystem II (PSII) reaction centers were sensitive to cerium-induced stress. Specifically, high concentrations of cerium damaged the oxygen evolving complex and PSII reaction center and suppressed electron transport at the donor and receptor side of the reaction center, influencing the absorption, transfer, and application of light energy in S. obliquus XJ002. In addition, we established a simple method to quantify the intracellular lipid content of S. obliquus XJ002, and the optimum staining conditions for Nile red were as follows: volume percentage of dimethyl sulfoxide was 2%, the concentration of Nile red was 2.0 µg·mL−1, and the staining time of Nile red was 5 min. The addition of cerium resulted in a significant increase in the total lipid content of XJ002. When the concentration of cerium was 50 mg·L−1, the total lipid content was 16.26% higher than the control group. This information will enhance our ability to utilize microelement fertilizer in biomass accumulation programs and will help to further reveal the key regulatory factors in the lipid metabolism, and would lay the foundation for promoting the research of microalgae bioenergy.
中文翻译：
外源铈对斜角栅藻 XJ002 体内叶绿素荧光和脂质产生的探测对光系统 II 的影响
铈是地壳中含量最丰富的稀土金属，化肥径流对水生生态系统产生有害影响。斜栅藻是一种富含油脂的微藻，生长迅速，对多种毒素敏感。鉴于微藻是富营养化和毒性应激的有用指标，发现较低浓度的铈（0.50-5.00 mg·L -1）刺激藻类生长并增加叶绿素 a 含量，而较高浓度（高于 50.00 mg·L -1 ) 对藻类生长和叶绿素 a 含量有抑制作用。藻类生长速度和叶绿素a含量在铈浓度为5.00 mg·L -1时达到峰值. 光系统 II (PSII) 反应中心的供体侧和受体侧都对铈诱导的应力敏感。具体而言，高浓度的铈破坏了析氧复合物和PSII反应中心，抑制了反应中心供体和受体侧的电子传输，影响了S. obliquus XJ002对光能的吸收、转移和应用。此外，我们建立了一种简单的方法来定量测定斜方链球菌 XJ002 细胞内脂质含量，尼罗红的最佳染色条件为：二甲基亚砜体积百分比为 2%，尼罗红浓度为 2.0 µg·毫升-1, 尼罗红染色时间为 5 min。添加铈导致 XJ002 的总脂质含量显着增加。当铈浓度为50 mg·L -1时，总脂质含量比对照组高16.26%。这些信息将提高我们在生物质积累项目中利用微量元素肥料的能力，有助于进一步揭示脂质代谢的关键调控因素，为推进微藻生物能源的研究奠定基础。
更新日期：2020-09-24
中文翻译：
外源铈对斜角栅藻 XJ002 体内叶绿素荧光和脂质产生的探测对光系统 II 的影响
铈是地壳中含量最丰富的稀土金属，化肥径流对水生生态系统产生有害影响。斜栅藻是一种富含油脂的微藻，生长迅速，对多种毒素敏感。鉴于微藻是富营养化和毒性应激的有用指标，发现较低浓度的铈（0.50-5.00 mg·L -1）刺激藻类生长并增加叶绿素 a 含量，而较高浓度（高于 50.00 mg·L -1 ) 对藻类生长和叶绿素 a 含量有抑制作用。藻类生长速度和叶绿素a含量在铈浓度为5.00 mg·L -1时达到峰值. 光系统 II (PSII) 反应中心的供体侧和受体侧都对铈诱导的应力敏感。具体而言，高浓度的铈破坏了析氧复合物和PSII反应中心，抑制了反应中心供体和受体侧的电子传输，影响了S. obliquus XJ002对光能的吸收、转移和应用。此外，我们建立了一种简单的方法来定量测定斜方链球菌 XJ002 细胞内脂质含量，尼罗红的最佳染色条件为：二甲基亚砜体积百分比为 2%，尼罗红浓度为 2.0 µg·毫升-1, 尼罗红染色时间为 5 min。添加铈导致 XJ002 的总脂质含量显着增加。当铈浓度为50 mg·L -1时，总脂质含量比对照组高16.26%。这些信息将提高我们在生物质积累项目中利用微量元素肥料的能力，有助于进一步揭示脂质代谢的关键调控因素，为推进微藻生物能源的研究奠定基础。
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