Study conducted to evaluate the impact of dietary incorporation of chitosan nanoparticles (CsNPs) bio-synthesis and Spirulina platensis on the growth performance and body composition of intensive cultured fingerling of black tiger shrimp (Penaeus monodon) in addition to the effect of the diets on heavy metals concentrations in water of culture cages. Six diets were prepared to contain approximately 44% crude protein and 499 k cal./100 g diet and supplemented by S. platensis and chitosan as follow: T1: control, T2: 0.05% S. platensis, T3: 0.1% S. platensis, T4: 1 ml chitosan, T5: 1 ml chitosan and 0.05% S. platensis and T6: 1 ml chitosan and 0.1% S. platensis. Shrimp fingerlings (1.24 ± 0.01 g) were randomly distributed in 12 hapas at stocking density of 20 fingerlings for each. The hapas were divided into 6 replicate groups and placed in cages. Each group received a kind of the experimental diet twice daily at feeding rate of 5% shrimp biomass for 12 weeks. The results indicated that, the growth parameters; final body weight (FBW g), weight gain (WG g), specific growth rate (SGR%) and survival rate (SR%) were improved significantly all trials compared to the control group. The best values for these parameters were recorded in the group of T5 and T6 without significant differences between them. Shrimp body composition showed the highest protein and fat contents for the group of T5 and T6 with significant differences (P < 0.05) among all treatments. No significant differences were noted for the heavy metals concentrations between all treatments. In conclusion, the incorporation of chitosan nanoparticles (CsNPs) and S. platensis attained considerable improvement in growth performance of black tiger shrimp and further investigations are recommended to optimize the level of incorporation in the diets for shrimp species.

进行研究以评估日粮中加入壳聚糖纳米颗粒（CsNPs）生物合成和螺旋藻对黑虎虾（对虾）精养鱼种的生长性能和身体组成的影响，以及日粮对沉重饲料的影响养殖笼中水中的金属浓度。制备六种日粮含有约44％的粗蛋白和499ķcal./100克的饮食和通过补充螺旋藻和脱乙酰壳多糖如下：T1：控制，T2：0.05％螺旋藻，T3：0.1％螺旋藻，T4：1 ml壳聚糖，T5：1 ml壳聚糖和0.05％的链球菌和T6：1 ml壳聚糖和0.1％的链球菌。虾鱼种（1.24±0.01 g）以20种鱼种的放养密度随机分布在12种情况下。将这些疾病分为6个重复组，并放在笼子里。每组每天两次以5％虾生物量的饲喂率接受一种实验性饮食，持续12周。结果表明，生长参数; 与对照组相比，所有试验的最终体重（FBW g），体重增加（WG g），比生长率（SGR％）和存活率（SR％）均有显着改善。这些参数的最佳值记录在T5和T6组中，两者之间没有显着差异。虾的身体成分显示，T5和T6组的蛋白质和脂肪含量最高，所有处理之间差异显着（P <0.05）。所有处理之间的重金属浓度均无显着差异。总之，壳聚糖纳米粒子（CsNPs）和S. platensis在黑虎虾的生长性能方面取得了显着改善，建议进一步研究以优化虾类饲料中的掺入水平。