The biggest challenge to apply Anammox to treat wastewater with elevated salt content is the inhibitory effect of salinity on freshwater Anammox bacteria (FAB). Most of the research into salinity inhibition has focused on the osmotic pressure effect, while the inhibitory effect and its mechanisms induced by ion composition are poorly understood. In this study, the individual and combined effect of NaCl, KCl and Na₂SO₄ on FAB (>99% belonging to Ca. Brocadia genera) were systematically investigated by batch tests. The corresponding responses of mRNA abundance of three functional genes (including nitrite reductase gene (nirS), hydrazine synthase gene (hzsB) and hydrazine dehydrogenase gene (hdh)) under different salt conditions were analyzed. The results indicated that NaCl, KCl and Na₂SO₄ have different inhibition effects, with the 50% inhibition at 0.106, 0.096 and 0.063 M, respectively. The combined inhibition of NaCl+KCl and NaCl+Na₂SO₄ on FAB were both synergistic; while the combined inhibition of NaCl+KCl+Na₂SO₄ was additive. The responses of mRNA (of genes: nirS, hzsB and hdh) suggested NaCl inhibited the transport of ammonium; Na₂SO₄ inhibited both nitrite and ammonium transport; high salinity inhibited functional enzyme activity. These results suggest both ionic stress and ion composition contributed to the observed inhibition.

将Anammox处理含盐量较高的废水所面临的最大挑战是盐度对淡水Anammox细菌（FAB）的抑制作用。关于盐度抑制的大多数研究都集中在渗透压效应上，而对离子组成引起的抑制作用及其机理了解得很少。在这项研究中，通过批处理系统地研究了NaCl，KCl和Na ₂ SO ₄对FAB（> 99％属于Ca. Brocadia属）的单独作用和联合作用。三种功能基因（亚硝酸还原酶基因（nirS），肼合酶基因（hzsB）和肼脱氢酶基因（hdh）的mRNA丰度的相应反应。））在不同的盐条件下进行了分析。结果表明，NaCl，KCl和Na ₂ SO ₄具有不同的抑制作用，分别在0.106、0.096和0.063 M处有50％的抑制作用。NaCl + KCl和NaCl + Na ₂ SO ₄对FAB的联合抑制作用是协同的。NaCl + KCl + Na ₂ SO ₄的综合抑制作用是累加的。mRNA（基因：nirS，hzsB和hdh）的反应表明，NaCl抑制了铵的转运。Na ₂ SO ₄抑制亚硝酸盐和铵的运输；高盐度抑制了功能酶的活性。这些结果表明离子应力和离子组成均对观察到的抑制作用有贡献。