In this work, an electrochemiluminescence resonance energy transfer (ECL-RET) system was established based on the modified graphite phase carbon nitride to detect brombuterol residues in food. The ultrasonic-assisted acidification exfoliation modification improved the conductivity and specific surface area of the graphite phase carbon nitride (g-C₃N₄). In addition, the carboxylated g-C₃N₄ nanosheets as ECL donors and the Au–Ag alloy nanoparticles as ECL acceptors could respectively directly carry antigen and antibody. Therefore, the trouble of introducing additional bridge molecules was avoided. A competitive immunoassay strategy was used for the detection of brombuterol, where brombuterol in the sample would compete with the coating antigen for the limited binding sites on antibody. The proposed ECL immunosensor for brombuterol detection exhibited high sensitivity with a wide linear range from 0.001 ng mL⁻¹ to 1000 ng mL⁻¹ and a low detection limit at 0.31 pg mL⁻¹. This work adopts a very simple way to design the sensor without losing its sensitivity, bringing convenience to its possible future applications.

在这项工作中，建立了基于改性石墨相氮化碳的电化学发光共振能量转移（ECL-RET）系统，以检测食品中的溴酚含量。超声辅助酸化剥落改性可改善石墨相氮化碳（gC ₃ N ₄）的电导率和比表面积。另外，羧化的gC ₃ N ₄作为ECL供体的纳米片和作为ECL受体的Au-Ag合金纳米片可以分别直接携带抗原和抗体。因此，避免了引入额外的桥分子的麻烦。竞争性免疫测定策略用于检测溴苯丁胺醇，其中样品中的溴苯丙胺醇将与包被抗原竞争抗体上有限的结合位点。所提出的用于溴丁固醇检测的ECL免疫传感器显示出高灵敏度，线性范围从0.001 ng mL ^-1到1000 ng mL ^-1，检测限低，为0.31 pg mL ^-1。这项工作采用了一种非常简单的方法来设计传感器，而又不损失其灵敏度，为将来可能的应用带来了便利。