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Stimulation of the phenylpropanoid pathway and antioxidant capacities by biotic and abiotic elicitation strategies in common bean (Phaseolus vulgaris) sprouts
Process Biochemistry ( IF 3.7 ) Pub Date : 2021-01-01 , DOI: 10.1016/j.procbio.2020.09.027
Josephine Oforiwaa Ampofo , Michael Ngadi

Abstract This study evaluated the impact of different concentrations of glutamic acid (1, 3, 5 and 7 mM) and sodium chloride (NaCl- 100, 200, 300 and 400 mM) elicitations on the biosynthesis of phenolic compounds and their antioxidant properties in common bean sprouts. Results showed that high glutamic acid concentrations (5 and 7 mM) elicited maximum levels of stress markers (H2O2, catalase and peroxidase), phenylpropanoid triggering enzymes (phenylalanine ammonia-lyase and tyrosine ammonia-lyase), and subsequent elevation of phenolic compounds. Although stress markers were elevated, it recorded no negative influence on the sprouting potential of common beans. Also, maximum antioxidant capacities were observed with phenolic compounds extract elicited with 5 mM glutamic acid at 96 h of sprouting, over their NaCl and control counterparts. Multiple quadratic regression studies led to the conclusion that, for optimum biosynthesis of phenolic compounds, synergistic activities of phenylalanine ammonia-lyase and tyrosine ammonia-lyase are required rather than in their isolated catalytic activities.

中文翻译:

通过生物和非生物诱导策略刺激普通豆 (Phaseolus vulgaris) 芽的苯丙烷途径和抗氧化能力

摘要 本研究评估了不同浓度的谷氨酸(1、3、5 和 7 mM)和氯化钠(NaCl-100、200、300 和 400 mM)诱导物对酚类化合物生物合成及其共同抗氧化特性的影响。豆芽。结果表明,高谷氨酸浓度(5 和 7 mM)会引发最高水平的应激标志物(H2O2、过氧化氢酶和过氧化物酶)、苯丙素触发酶(苯丙氨酸解氨酶和酪氨酸解氨酶),以及随后的酚类化合物升高。尽管压力标记升高,但它对普通豆的发芽潜力没有负面影响。此外,与 NaCl 和对照对应物相比,在发芽 96 小时时用 5 mM 谷氨酸引发的酚类化合物提取物具有最大的抗氧化能力。
更新日期:2021-01-01
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