Abstract A compact and low-cost system capable of Digital Holographic Microscopic (DHM) imaging of immobilized micro-particles is presented. The proposed design, integration, and manufacturing can lead to wider use and increased accessibility of this device for research, education, and biomedical applications. The optical tweezer system for particle immobilization is built using the optical pickup unit taken from a DVD burner. DHM is integrated into the system through the addition of a glass plate which enables one arm of the system to operate as a lateral shearing digital holographic interferometer. The operation of the system is confirmed by trapping, quantitative phase imaging and analysis of polystyrene micro-spheres for which the corner frequency measurements, carried using an in-house developed low-cost Quadrant Photodiode, compare favorably with simulated results. Furthermore, the system is illustrated for trapping and simultaneous quantitative phase imaging of micro-particles, including biological specimen such as red blood cells. The use of a low-cost easily implementable DHM integrated optical tweezer system provides vast information content, which enable in-depth analysis of immobilized micro-particles.

中文翻译：

---

用于固定微粒数字全息显微镜的紧凑型低成本仪器

摘要 提出了一种能够对固定化微粒进行数字全息显微 (DHM) 成像的紧凑且低成本的系统。所提议的设计、集成和制造可以导致该设备在研究、教育和生物医学应用中的更广泛使用和更高的可访问性。用于粒子固定的光学镊子系统是使用从 DVD 刻录机中取出的光学拾取单元构建的。DHM 通过添加玻璃板集成到系统中，使系统的一个臂能够作为横向剪切数字全息干涉仪运行。该系统的运行通过聚苯乙烯微球的捕获、定量相位成像和分析得到确认，转角频率测量使用内部开发的低成本象限光电二极管进行，与模拟结果相比较。此外，该系统被说明用于微粒的捕获和同时定量相位成像，包括生物样本，如红细胞。使用低成本、易于实施的 DHM 集成光镊系统提供了大量的信息内容，可以对固定化的微粒进行深入分析。