Vibrio cholerae, causative agent of the water-borne disease cholera still threatens a large proportion of world's population. The major biotypes of the pathogen are classical and El Tor. There have been recent reports of variant V. cholerae strains circulating around the world. In the present study, the epidemiological status of V. cholerae strains circulating in the country over a decade was assessed. Also, a comprehensive analysis of the difference in pathogenicity between the different biotypes of V. cholerae strains was evaluated both in-vitro and in-vivo. The amount of CT produced by different biotypes of V. cholerae strains were analyzed by GM₁ ELISA and the probable reasons for the difference in toxin production was discussed. MLST analysis grouped the isolates into a single Sequence Type (ST 69) whereas PFGE analysis clustered the isolates into ten different pulsotypes revealing molecular diversity. The circulating strains were identified to produce cholera toxin and CT mRNA intermediate to the classical and prototype El Tor strains. Also, the circulating strains were identified to possess four ToxR binding sequences. In-vivo pathogenicity analysis by rabbit ileal loop fluid accumulation assay revealed the Haitian variant strains to be more hyperemic than the prototype strains.

霍乱弧菌是霍乱水传播疾病的病原体，仍然威胁着世界上很大一部分人口。病原体的主要生物类型是经典和El Tor。最近有报道说，霍乱弧菌变异株在世界范围内传播。在本研究中，评估了该国十多年来传播的霍乱弧菌菌株的流行病学状况。此外，在体外和体内均评估了霍乱弧菌不同生物型之间致病性差异的综合分析。GM ₁分析了不同生物型霍乱弧菌菌株产生的CT量讨论了ELISA和毒素产生差异的可能原因。MLST分析将这些分离物归为一个单一的序列类型（ST 69），而PFGE分析则将这些分离物归为十种不同的脉冲型，揭示了分子多样性。经鉴定，循环菌株可产生霍乱毒素和CT mRNA，介于经典和原型El Tor菌株之间。另外，鉴定出循环菌株具有四个ToxR结合序列。通过兔回肠loop液积聚试验进行的体内致病性分析表明，海地变异株比原型株具有更高的充血性。