Staphylococcus aureus is emerging as complicated pathogen because of its wide-ranging origin, multiple variants, and compromised antibiotic susceptibilities. Current study was planned to find lineage of hospital acquired methicillin resistant Staphylococcus aureus (HA-MRSA), and its comparative phenotypic clinico-epidemiology with vancomycin resistant S. aureus (VRSA). A total of (n = 200) samples were aseptically collected from wound, nose, and cerebrospinal fluid of patients from metropolitan and rural background hospitals along with on spot filling in of questionnaire. Phylogenetic analysis of HA-MRSA was identified by targeting mecA gene in S. aureus. At optimal tree branch length of 1.91 and evolutionary distance 0.1, high level sequence similarity (97%–99%) was observed with different strains of S. aureus isolated from both human and animal. Non-descriptive statistics at 5% probability found 61% S. aureus, while 43.44% of them were HA-MRSA, 92.62% VRSA, and 42.62% were both MRSA and VRSA. Among the assumed risk factors, use of antibiotics, venous catheterization, chronic disease, pre-hospital visits, and ICU admitted patients showed significant association (p < 0.05) with pathogen. HA-MRSA was 37.50%, 80%, and 37.50% sensitive to chloramphenicol, gentamicin, and oxacillin, respectively. While <50% of VRSA sensitive against oxacillin, enoxacin, and chloramphenicol. A significant difference (p < 0.05) of percentage responses of MRSA and VRSA at resistant, intermediate, and sensitive cadre against all antibiotics except chloramphenicol was obvious in this study. The Current study concluded higher prevalence of MRSA & VRSA, significant association of risk factors, limiting antibiotic susceptibility profile, and genetic transfer at animal & human interface suggestive of further studies cum preventive strategies to be planned.

金黄色葡萄球菌由于其来源广泛，多种变异以及对抗生素的敏感性降低而成为复杂的病原体。目前的研究计划是寻找医院获得的耐甲氧西林金黄色葡萄球菌（HA-MRSA）的血统，以及与耐万古霉素的金黄色葡萄球菌（VRSA）的比较表型临床流行病学。从大城市和农村背景医院的患者的伤口，鼻子和脑脊液中无菌采集了总共（n = 200）个样本，并当场填写了问卷。通过靶向金黄色葡萄球菌mecA基因鉴定HA-MRSA的系统发育分析。在最佳树枝长度为1.91且进化距离为0.1的情况下，从人和动物分离出的不同金黄色葡萄球菌菌株均观察到高水平的序列相似性（97％–99％）。非描述性统计学以5％的概率发现金黄色葡萄球菌为61％，而HA-MRSA为43.44％，VRSA为92.62％，MRSA和VRSA均为42.62％。在假定的危险因素中，抗生素的使用，静脉导管插入，慢性疾病，院前就诊和ICU入院患者显示出与病原体的显着相关性（p <  0.05）。HA-MRSA对氯霉素，庆大霉素和奥沙西林的敏感度分别为37.50％，80％和37.50％。而<50％的VRSA对奥沙西林，依诺沙星和氯霉素敏感。显着差异（ 在本研究中，MRSA和VRSA在耐药，中级和敏感干部对除氯霉素以外的所有抗生素的反应百分率均很明显（p <0.05）。本研究得出的结论是，MRSA和VRSA的患病率较高，危险因素之间的显着相关性，抗生素敏感性分布受到限制以及动物和人与人之间的基因转移，这表明需要进行进一步的研究并制定计划的预防策略。