The effects of pharmacological stimulation of skin ion channels TRPA1, TRPM8, TRPV1 on the immune response are presented. These effects are compared with the effects of different types of temperature exposures - skin cooling, deep cooling, and deep heating. This analysis allows us to clear the differences in the influence on the immune response of thermosensitive ion channels localized in the skin; (2) whether the changes in the immune response under temperature exposures are due to these thermosensitive ion channels. Experiments were performed on Wistar rats. For stimulation of TRPM8 ion channel, an application to the skin of 1% menthol was used, for TRPA1 - 0.04% allylisotiocianate, and for TRPV1 - capsaicin in a concentration of 0.001.The antigen binding in the spleen was two-times stimulated by activation of the cold-sensitive ion channel TRPM8 and much weaker by activation of warm-sensitive TRPV1 (by 15%), and another cold-sensitive ion channel TRPA1 (by 40%). Only the stimulation of TRPA1 significantly (by 140%) increased antibody formation in the spleen, while TRPM8 had practically no effect on this process, and activation of TRPV1 significantly (by 60%) inhibited antibody formation. Stimulation of the TRPM8 ion channel significantly (by 60%) reduced the level of IgG in the blood, which is believed to control of infectious diseases.The obtained results show that pharmacological activation of the skin TRPA1, TRPM8, TRPV1 ion channels can differently affect the immune system. At the epicenter of changes there were the antigen binding and antibody formation in the spleen, as well as the level of IgG in the blood. Exactly stimulation of the TRPM8 ion channel determines the changes in the immune response when only the skin is cooling, while at deep body heating, the changes in the immune response are mostly determined by the activation of the skin TRPV1 ion channel.

中文翻译：

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SKIN ION 通道 TRPM8、TRPV1 和 TRPA1 激活对免疫反应的影响。冷热暴露效果比较

介绍了皮肤离子通道 TRPA1、TRPM8、TRPV1 的药理刺激对免疫反应的影响。将这些影响与不同类型的温度暴露的影响进行比较 - 皮肤冷却、深度冷却和深度加热。该分析使我们能够明确位于皮肤中的热敏离子通道对免疫反应影响的差异；(2) 温度暴露下免疫反应的变化是否是由这些热敏离子通道引起的。实验是在 Wistar 大鼠上进行的。为了刺激 TRPM8 离子通道，将 1% 薄荷醇应用于皮肤，对于 TRPA1 - 0.04% 烯丙基异硫氰酸酯，对于 TRPV1 - 辣椒素，浓度为 0.001。脾脏中的抗原结合受到冷敏感离子通道 TRPM8 的激活的两次刺激，而温敏性 TRPV1（15%）和另一个冷敏感离子通道 TRPA1（40%）的激活则更弱。 . 只有 TRPA1 的刺激显着（140%）增加了脾脏中的抗体形成，而 TRPM8 对该过程几乎没有影响，并且 TRPV1 的激活显着（60%）抑制了抗体的形成。TRPM8 离子通道的刺激显着（降低 60%）降低了血液中的 IgG 水平，这被认为可以控制传染病。 获得的结果表明皮肤 TRPA1、TRPM8、TRPV1 离子通道的药理学激活可以不同地影响免疫系统。在变化的中心是脾脏中的抗原结合和抗体形成，以及血液中的 IgG 水平。TRPM8离子通道的确切刺激决定了只有皮肤冷却时免疫反应的变化，而在身体深部加热时，免疫反应的变化主要是由皮肤TRPV1离子通道的激活决定的。