The versatility of Clustered Regularly Interspaced Short Palindromic Repeats/Cas (CRISPR/Cas) genome editing tool ushered biologists into an exciting era of editing genomes with great efficiency and at a pace that was never imagined before. Though the CRISPR/Cas genome editing was developed after Zinc Finger Nucleases (ZFNs) and Transcription activator-like effector nucleases (TALENs), it is more popular and successful than these genome editing systems. The advent of targetable nucleases such as Cas9 has enabled manipulation of genomes in an accurate and precise manner. The CRISPR/Cas system of editing plant genomes has technical and economical advantages over conventional breeding methods. It has led to the development of traits within plant genomes that fulfill the needs of mankind. Advent of innovative procedures have paved the way for effective and efficient genome editing that has revolutionized genetic aspects and meets the safety regulations toward development of crops. The present review highlights the critical aspects of employing CRISPR/Cas for editing plant genomes in comparison with previously known editing approaches, such as ZFNs and TALENs. The study includes descriptive information on the approaches, procedural programs and applications in editing plant genomes for improving traits such as crop yield, resistance against emerging pathogens, abiotic stresses and herbicide tolerance thereof in the present-day world.

成簇规则间隔短回文重复序列/Cas (CRISPR/Cas) 基因组编辑工具的多功能性使生物学家进入了一个激动人心的时代，以前所未有的速度和高效率编辑基因组。尽管 CRISPR/Cas 基因组编辑是在锌指核酸酶 (ZFNs) 和转录激活剂样效应核酸酶 (TALENs) 之后发展起来的，但它比这些基因组编辑系统更受欢迎和成功。可靶向核酸酶（例如 Cas9）的出现使得能够以准确和精确的方式操纵基因组。编辑植物基因组的 CRISPR/Cas 系统比传统育种方法具有技术和经济优势。它导致了满足人类需求的植物基因组特征的发展。创新程序的出现为有效和高效的基因组编辑铺平了道路，基因组编辑彻底改变了遗传方面并符合作物开发的安全法规。本综述强调了使用 CRISPR/Cas 编辑植物基因组与之前已知的编辑方法（如 ZFN 和 TALEN）相比的关键方面。该研究包括有关编辑植物基因组的方法、程序和应用的描述性信息，以改善当今世界的作物产量、对新兴病原体的抗性、非生物胁迫和除草剂耐受性等性状。本综述强调了使用 CRISPR/Cas 编辑植物基因组与之前已知的编辑方法（如 ZFN 和 TALEN）相比的关键方面。该研究包括有关编辑植物基因组的方法、程序和应用的描述性信息，以改善当今世界的作物产量、对新兴病原体的抗性、非生物胁迫和除草剂耐受性等性状。本综述强调了使用 CRISPR/Cas 编辑植物基因组与之前已知的编辑方法（如 ZFN 和 TALEN）相比的关键方面。该研究包括有关编辑植物基因组的方法、程序和应用的描述性信息，以改善当今世界的作物产量、对新兴病原体的抗性、非生物胁迫和除草剂耐受性等性状。