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Toxicological assessment and developmental abnormalities induced by butylparaben and ethylparaben exposure in zebrafish early-life stages
Environmental Toxicology and Pharmacology ( IF 4.3 ) Pub Date : 2020-09-24 , DOI: 10.1016/j.etap.2020.103504
C. Merola , O. Lai , A. Conte , G. Crescenzo , T. Torelli , M. Alloro , M. Perugini

Toxicological effects of butylparaben (BuP) and ethylparaben (EtP) on zebrafish (Danio rerio) early-life stages are not well established. The present study evaluated, using zebrafish embryos and larvae, the toxicity of BuP and EtP through benchmark dose (BMD) approach. BuP was more toxic than EtP to zebrafish larvae. In fact, Lethal Concentration 50 (LC50) values at 96 h post-fertilization (hpf) for BuP and EtP were 2.34 mg/L and 20.86 mg/L, respectively. Indeed, BMD confidence interval (lower bound (BMDL) - upper bound (BMDU) was 0.91–1.92 mg/L for BuP and 10.8–17.4 mg/L for EtP. Zebrafish embryos exposed to 1 mg/L, 2.5 mg/L of BuP and 5 mg/L, 10 mg/L, 20 mg/L, 30 mg/L of EtP showed several developmental abnormalities and teratological effects compared to negative control. Exposed zebrafish developed reduced heartbeat, reduction in blood circulation, blood stasis, pericardial edema, deformed notochord and misshaped yolk sac. Embryos exposed to the highest concentrations of the chemicals (2.5 mg/L of BuP, 10 mg/L, 20 mg/L and 30 mg/L of EtP) showed the developmental abnormalities at 48 hpf while those treated with 1 mg/L of BuP and 10 mg/L of EtP reported behavioral changes at 72 hpf, including trembling of head, pectoral fins and spinal cord. This research identified the lethal and sublethal effects of BuP and EtP in zebrafish early-life stages and could be helpful to elucidate the developmental pathways of toxicity of parabens.



中文翻译:

斑马鱼生命早期阶段由对羟基苯甲酸丁酯和对羟基苯甲酸乙酯暴露引起的毒理学评估和发育异常

对羟基苯甲酸丁酯(BuP)和对羟基苯甲酸乙酯(EtP)对斑马鱼(Danio rerio)的毒理作用)早期阶段尚不完善。本研究使用斑马鱼胚胎和幼虫通过基准剂量(BMD)方法评估了BuP和EtP的毒性。BuP比EtP对斑马鱼幼虫的毒性更大。实际上,BuP和EtP在受精后96 h(hpf)的致死浓度50(LC50)值分别为2.34 mg / L和20.86 mg / L。实际上,BuP的BMD置信区间(下限(BMDL)-上限(BMDU))为0.91–1.92 mg / L,EtP为10.8–17.4 mg / L。斑马鱼胚胎暴露于1 mg / L,2.5 mg / L与阴性对照组相比,BuP和5 mg / L,10 mg / L,20 mg / L,30 mg / L的EtP表现出一些发育异常和致畸作用,暴露的斑马鱼出现心跳减少,血液循环减少,血瘀,心包膜减少水肿,脊索畸形和卵黄囊变形。暴露于最高浓度化学物质(2.5 mg / L的BuP,10 mg / L,20 mg / L和30 mg / L的EtP)的胚胎在48 hpf时出现发育异常,而用1 mg / L的EtP处理BuP和10 mg / L EtP在72 hpf时报告了行为变化,包括头部,胸鳍和脊髓发抖。这项研究确定了BuP和EtP在斑马鱼生命早期阶段的致死作用和亚致死作用,可能有助于阐明对羟基苯甲酸酯毒性的发育途径。

更新日期:2020-10-04
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