Misuse and abuse of prescription drugs has become a global health problem in the last few years as the illicit drug cartels have grown exponentially. The non-medical use of prescribed medications, such as benzodiazepines, synthetic opioids, stimulants and tranquilizers, has increased rapidly due to their easy availability. Nowadays benzodiazepines are not only misused/abused, but also play a significant part in facilitating various crimes such as robbery, date rapes, sexual harassment, which has made its forensic examination important and critical. Considering the gravity of these crimes, an attempt has been made to develop and validate simultaneous quantification method for seven benzodiazepines (clonazepam, lorazepam, alprazolam, diazepam, flurazepam, oxazepam and nitrazepam) using high-performance thin-layer chromatography–mass spectrometry (HPTLC–MS) from urine. Quantitative analysis of clonazepam, lorazepam, alprazolam, diazepam, flurazepam, oxazepam and nitrazepam was carried out using HPTLC–MS. MS operating in selective ion-monitoring mode was attempted for the accurate identification of the drugs under study. Small-volume liquid extraction technique using diethyl ether–chloroform (80:20, v/v) was used for the extraction of urine. Pre-coated HPTLC (silica gel G 60 F₂₅₄) plates were developed using the mobile-phase chloroform–glacial acetic acid (9:1, v/v). The screening of HPTLC plates was carried out by ultraviolet light, and the m/z ratio of drugs was obtained by extracting spots from plates using a TLC–MS interface. Method validation was carried out according to the International Council for Harmonisation guidelines. The current HPTLC–MS method is simple, sensitive, precise and accurate, and it can be used for the detection and quantitative estimation of benzodiazepines in urine.

近年来，随着非法药物卡特尔成倍增长，滥用和滥用处方药已成为全球性的健康问题。处方药物（如苯二氮卓类药物，合成阿片类药物，兴奋剂和镇定剂）的非医疗用途由于易于获取而迅速增加。如今，苯二氮卓不仅被滥用/滥用，而且在促进各种犯罪（例如抢劫，强奸，性骚扰）中起着重要作用，这使其法医检查变得十分重要和关键。考虑到这些罪行的严重性，已尝试开发和验证同时对七个苯二氮卓类药物（氯硝西am，劳拉西m，阿普唑仑，地西epa，氟拉西m，使用高效薄层色谱-质谱法（HPTLC-MS）从尿液中分离出奥沙西m和硝西epa。使用HPTLC-MS进行了氯硝西am，劳拉西m，阿普唑仑，地西epa，氟拉西，奥沙西nitr和硝西Quant的定量分析。尝试以选择性离子监测模式运行的MS，以准确鉴定正在研究的药物。使用二乙醚-氯仿（80：20，v / v）的小体积液体提取技术提取尿液。预涂HPTLC（硅胶G 60 F 使用二乙醚-氯仿（80：20，v / v）的小体积液体提取技术提取尿液。预涂HPTLC（硅胶G 60 F 使用二乙醚-氯仿（80：20，v / v）的小体积液体提取技术提取尿液。预涂HPTLC（硅胶G 60 F使用流动相氯仿-冰醋酸（9：1，v / v）开发了₂₅₄）板。HPTLC板的筛选是通过紫外光进行的，药物的m / z比是通过使用TLC-MS接口从板中提取斑点来获得的。方法验证是根据国际协调理事会的指导原则进行的。当前的HPTLC-MS方法简便，灵敏，精确，准确，可用于尿液中苯并二氮杂的检测和定量评估。