Abstrast —A novel 2-phenyl-1,2,3-triazole derived fluorescent probe HPTC ((2-hydroxybenzylidene)-2-phenyl-2 H -1,2,3-triazole-4-carbohydrazide) has been designed and synthesized for recyclable detection of Al 3+ in DMSO/Tris-HCl (pH 7.4) 1 : 100 v/v with high sensitivity and selectivity. A fluorescence enhancement of 97 folds at 457 nm can be realized upon addition of 10.0 equiv. Al 3+ with excitation at 375 nm. The binding constant of HPTC with Al 3+ and the detection limit of HPTC toward Al 3+ were 1.67 × 10 5 M –1 and 8.875 × 10 –8 M, respectively. In addition, the probe HPTC can detect Al 3+ repeatedly in DMSO/Tris-HCl (pH 7.4) 1 : 100 v/v and the detection effect is not changed. The probe HPTC showed an excellent detection ability in a wide pH range from 3 to 10 and also in living cells. Furthermore, the Al 3+ sensing mechanism was confirmed based on Job’s plot, 1 H NMR titration, HR-MS, and DFT studies. Finally, HPTC -doped agarose hydrogel experiment suggests that the HPTC can recognize the Al 3+ in solid state.

中文翻译：

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一种新型2-苯基-1,2,3-三唑衍生荧光探针可回收检测水性介质中的Al3+及其应用

Abstrast——设计并合成了一种新型2-苯基-1,2,3-三唑衍生荧光探针HPTC（(2-hydroxybenzylidene)-2-phenyl-2 H -1,2,3-triazole-4-carbohydrazide）用于 DMSO/Tris-HCl (pH 7.4) 1 : 100 v/v 中 Al 3+ 的可回收检测，具有高灵敏度和选择性。添加 10.0 当量后，可在 457 nm 处实现 97 倍的荧光增强。Al 3+ 激发波长为 375 nm。HPTC 与Al 3+ 的结合常数和HPTC 对Al 3+ 的检测限分别为1.67 × 10 5 M –1 和8.875 × 10 –8 M。此外，探针HPTC可以在DMSO/Tris-HCl (pH 7.4) 1 : 100 v/v 中重复检测Al 3+ 且检测效果不变。HPTC 探针在 3 到 10 的宽 pH 范围内以及在活细胞中均显示出出色的检测能力。此外，Al 3+ 传感机制根据乔布斯图、 1 H NMR 滴定、HR-MS 和 DFT 研究得到确认。最后，HPTC掺杂的琼脂糖水凝胶实验表明HPTC可以识别固态的Al 3+ 。