Irradiance-driven 20-hydroxyecdysone production and morphophysiological changes in Pfaffia glomerata plants grown in vitro,Protoplasma

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Irradiance-driven 20-hydroxyecdysone production and morphophysiological changes in Pfaffia glomerata plants grown in vitro
Protoplasma ( IF 2.5 ) Pub Date : 2020-09-25 , DOI: 10.1007/s00709-020-01558-1
Tatiane Dulcineia Silva ₁ , Diego Silva Batista ₂ , Kamila Motta Castro ₁ , Evandro Alexandre Fortini ₁ , Sérgio Heitor Sousa Felipe ₁ , Amanda Mendes Fernandes ₁ , Raysa Mayara Jesus Sousa ₃ , Kristhiano Chagas ₁ , José Victor Siqueira da Silva ₁ , Ludmila Nayara Freitas Correia ₁ , Gabriela Torres-Silva ₁ , Letícia Monteiro Farias ₄ , Wagner Campos Otoni ₁

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Pfaffia glomerata possesses potential pharmacological and medicinal properties, mainly owing to the secondary metabolite 20-hydroxyecdysone (20E). Increasing production of biomass and 20E is important for industrial purposes. This study aimed to evaluate the influence of irradiance on plant morphology and production of 20E in P. glomerata grown in vitro. Nodal segments of accessions 22 and 43 (Ac22 and Ac43) were inoculated in culture medium containing MS salts and vitamins. Cultures were maintained at 25 ± 2 °C under a 16-h photoperiod and subjected to irradiance treatments of 65, 130, and 200 μmol m −2 s −1 by fluorescent lamps. After 30 days, growth parameters, pigment content, stomatal density, in vitro photosynthesis, metabolites content, and morphoanatomy were assessed. Notably, Ac22 plants exhibited 10-fold higher 20E production when cultivated at 200 μmol m −2 s −1 than at 65 μmol m −2 s −1 , evidencing the importance of light quantity for the accumulation of this metabolite. 20E production was twice as high in Ac22 as in Ac43 plants although both accessions responded positively to higher irradiance. Growth under 200 μmol m −2 s −1 stimulated photosynthesis and consequent biomass accumulation, but lowered carotenoids and anthocyanins. Furthermore, increasing irradiance enhanced the number of palisade and spongy parenchyma cells, enhancing the overall growth of P. glomerata . Graphical abstract

中文翻译：

辐照度驱动的 20-羟基蜕皮激素的产生和体外生长的 Pfaffia glomerata 植物的形态生理变化

Pfaffia glomerata 具有潜在的药理和药用特性，主要是由于次级代谢产物 20-羟基蜕皮激素 (20E)。增加生物质和 20E 的产量对于工业用途很重要。本研究旨在评估辐照度对体外生长的 P. glomerata 中植物形态和 20E 产量的影响。将第 22 和 43 号材料（Ac22 和 Ac43）的节段接种在含有 MS 盐和维生素的培养基中。培养物在 16 小时光周期下保持在 25 ± 2 °C，并通过荧光灯进行 65、130 和 200 μmol m -2 s -1 的辐照处理。30 天后，评估了生长参数、色素含量、气孔密度、体外光合作用、代谢物含量和形态解剖学。尤其，Ac22 植物在 200 μmol m -2 s -1 培养时的 20E 产量比在 65 μmol m -2 s -1 培养时高 10 倍，证明光量对于该代谢物的积累的重要性。Ac22 中的 20E 产量是 Ac43 植物的两倍，尽管两个种质都对更高的辐照度做出了积极响应。在 200 μmol m -2 s -1 下的生长刺激了光合作用和随之而来的生物量积累，但降低了类胡萝卜素和花青素。此外，增加辐照度增加了栅栏和海绵状薄壁组织细胞的数量，促进了球状假单胞菌的整体生长。图形概要 Ac22 中的 20E 产量是 Ac43 植物的两倍，尽管两个种质都对更高的辐照度做出了积极响应。在 200 μmol m -2 s -1 下的生长刺激了光合作用和随之而来的生物量积累，但降低了类胡萝卜素和花青素。此外，增加辐照度增加了栅栏和海绵状薄壁组织细胞的数量，促进了球状假单胞菌的整体生长。图形概要 Ac22 中的 20E 产量是 Ac43 植物的两倍，尽管两个种质都对更高的辐照度做出了积极响应。在 200 μmol m -2 s -1 下的生长刺激了光合作用和随之而来的生物量积累，但降低了类胡萝卜素和花青素。此外，增加辐照度增加了栅栏和海绵状薄壁组织细胞的数量，促进了球状假单胞菌的整体生长。图形概要

更新日期：2020-09-25

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