Abstract—Western blot and quantitative real-time PCR were used to study the content of the regulatory alpha subunit of hypoxia-inducible factor-1 (HIF1α), and the mRNA content of the HIF1-regulated genes lactate dehydrogenase A (ldha) and glucose-6-phosphate dehydrogenase (g6pd) in the hippocampus of 1-day old rat pups whose mothers were subjected to three sessions of severe damaging hypoxia on days 14–16 of pregnancy. Prenatal hypoxia caused a stable increase in HIF1α content, and also led to an increase in the ldha mRNA content and a decrease in the g6pd mRNA content. The results indicate that changes in hippocampal metabolism after prenatal hypoxia are stable and can cause disturbances in the energy metabolism of the brain in further ontogeny.

摘要—用Western印迹和定量实时PCR研究缺氧诱导因子-1（HIF1α）的调节α亚基的含量，以及HIF1调控的基因乳酸脱氢酶A（ldha）和葡萄糖的mRNA含量。1天大的幼仔海马中的-6-磷酸脱氢酶（g6pd），其母鼠在怀孕的14-16天经历了三节严重的破坏性缺氧。产前缺氧导致HIF1α含量稳定增加，还导致ldha mRNA含量增加和g6pd降低mRNA含量。结果表明，产前缺氧后海马代谢的变化是稳定的，并可在进一步的个体发育中引起大脑能量代谢的紊乱。