Abstract—Exosomes are extracellular vesicles that represent an important mode of intercellular communication. It is thought that exosomes can carry biologically active molecules associated with the progressive spreading of amyotrophic lateral sclerosis (ALS). ALS is a fatal neurodegenerative disease characterized by the progressive death of motor neurons. Angiogenin is likely to be important in the pathogenesis of ALS. This paper analyzes the content of angiogenin in blood plasma, CSF, and exosome fractions obtained from blood plasma and CSF in ALS. The study included 30 patients with ALS and 26 age and gender matched healthy controls. The relative amount of angiogenin in plasma and CSF was significantly higher than in exosome fractions (p < 0.05). A comparison of angiogenin content in plasma and exosome fraction revealed lower angiogenin levels in patients with ALS compared to healthy volunteers (p < 0.05), which may indicate a protective role of angiogenin. Higher concentrations of angiogenin in plasma and its exosome fractions tended to correlate with better functional states in patients. Therefore, a reduction of angiogenin levels in plasma and the exosome fraction from plasma may be a marker of disease progression, but these data need to be confirmed in a larger cohort.

摘要—外来体是细胞外囊泡，代表细胞间通讯的一种重要方式。据认为，外泌体可以携带与肌萎缩性侧索硬化症（ALS）的逐渐扩散有关的生物活性分子。ALS是一种致命的神经退行性疾病，其特征在于运动神经元的进行性死亡。血管生成素可能在ALS的发病机理中很重要。本文分析了血浆，CSF中血管生成素的含量，以及从ALS中血浆和CSF获得的外来体组分的含量。该研究包括30例ALS患者和26例年龄和性别匹配的健康对照。血浆和脑脊液中血管生成素的相对含量显着高于外体组分（p<0.05）。血浆和外泌体组分中血管生成素含量的比较显示，与健康志愿者相比，ALS患者的血管生成素水平较低（p <0.05），这可能表明血管生成素具有保护作用。血浆及其外泌体组分中较高的血管生成素浓度往往与患者更好的功能状态相关。因此，血浆中血管生成素水平的降低以及血浆中的外泌体分数可能是疾病进展的标志，但是这些数据需要在更大的队列中得到证实。