Silver nanoparticles conjugated MnFe-based Prussian blue analogue for voltammetric and impedimetric bioaptasensing of amifostine (ethyol),Microchimica Acta

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Silver nanoparticles conjugated MnFe-based Prussian blue analogue for voltammetric and impedimetric bioaptasensing of amifostine (ethyol)
Microchimica Acta ( IF 5.3 ) Pub Date : 2020-09-25 , DOI: 10.1007/s00604-020-04557-4
Saad A Alkahtani ₁

Affiliation

A novel bioaptasensing-based electrochemical method for determination of amifostine (AMF) is proposed. The electrochemical aptasensor is based on modification of a glassy carbon electrode with a nanocomposite consisting of silver nanoparticles @ MnFe Prussian blue analogue nanospheres (AgNPs@MnFePBA NS), followed by immobilization of aptamer via Ag-N bonds (aptamer/AgNPs@MnFePBA NS/GCE). Experimental parameters including pH, incubation time, and aptamer concentrations were optimized. Electrochemical impedance spectroscopy (EIS) and differential pulse voltammetric (DPV) techniques were utilized to quantify AMF. The anodic peak current (∆Ipa) and charge transfer resistance (∆Rct) differences increase in the presence of AMF. Under the optimal conditions, using the redox probe, the electrochemical aptasensor exhibited linear ranges of 0.34–45 nmol L−1 and 0.69–45 nmol L−1 with LODs of 0.11 nmol L−1 and 0.23 nmol L−1 for EIS and DPV, respectively. The aptasensor was used to determine AMF in human plasma and in the presence of interfering species with recoveries and RSDs in the range 97.8–103.2% and 2.2–4.2%, respectively. Graphical abstract Graphical abstract

中文翻译：

银纳米粒子结合基于锰铁的普鲁士蓝类似物，用于氨磷汀（乙醇）的伏安法和阻抗法生物适应传感

提出了一种用于测定氨磷汀 (AMF) 的新的基于生物适应性的电化学方法。电化学适体传感器基于用由银纳米颗粒@MnFe 普鲁士蓝模拟纳米球 (AgNPs@MnFePBA NS) 组成的纳米复合材料对玻碳电极进行改性，然后通过 Ag-N 键固定适体 (适体/AgNPs@MnFePBA NS/ GCE）。优化了实验参数，包括 pH、孵育时间和适体浓度。电化学阻抗谱 (EIS) 和差分脉冲伏安 (DPV) 技术被用来量化 AMF。阳极峰值电流 (ΔIpa) 和电荷转移电阻 (ΔRct) 差异在 AMF 存在下增加。在最佳条件下，使用氧化还原探针，电化学适体传感器的线性范围为 0。34-45 nmol L-1 和 0.69-45 nmol L-1，EIS 和 DPV 的 LOD 分别为 0.11 nmol L-1 和 0.23 nmol L-1。适体传感器用于测定人血浆中和存在干扰物质时的 AMF，回收率和 RSD 范围分别为 97.8-103.2% 和 2.2-4.2%。图形摘要图形摘要

更新日期：2020-09-25

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