Sogatella furcifera (Horvath) is a notorious pest in most rice-producing regions of Asia, and evidence of resistance of S. furcifera to various insecticides has been documented in many areas. Given that the risk of insecticide resistance has spread, prospective monitoring should be expedited. Diagnostic tools for the rapid and accurate assessment of insecticide resistance are urgently needed to implement pest control and effective insecticide resistance management strategies. In the present study, a diagnostic kit based on the glass vial bioassay for the rapid detection of resistance to imidacloprid, nitenpyram, clothianidin, dinotefuran, thiamethoxam, isoprocarb and chlorpyrifos in S. furcifera was developed. The detection results can be obtained in 1 h, and the determination of ≥ 90% mortality indicates sensitivity to insecticides. The insecticide resistance levels diagnosed by the kit were consistent with the results of the rice seedling dip method in field populations of S. furcifera. Moreover, the accuracy of the diagnosis of nitenpyram and chlorpyrifos resistance was further verified by field trials. The insecticide resistance diagnostic kit for S. furcifera, as a user-friendly tool (portable, short detection time), can be easily popularized. Its excellent performance qualifies the kit as a reliable screening tool to determine effective insecticides and a convenient addition to integrated pest management programs.

Sogatella furcifera（Horvath）是亚洲大多数水稻生产地区的一个臭名昭著的害虫，并且在许多地区都有记载S. furcifera对各种杀虫剂具有抗性的证据。鉴于对杀虫剂产生抗药性的风险已经扩散，应加快前瞻性监测。为了实施害虫控制和有效的杀虫剂抗性管理策略，迫切需要用于快速准确评估杀虫剂抗性的诊断工具。在本研究中，基于玻璃小瓶生物测定法的诊断试剂盒可用于快速检测对S. furcifera中的吡虫啉，尼替吡兰，可比丁，地替呋喃，噻虫嗪，异丙威和毒死rif的耐药性已开发。检测结果可在1小时内获得，并且死亡率≥90％的测定表明对杀虫剂敏感。该试剂盒诊断的杀虫剂抗性水平与稻瘟病菌田间种群的水稻浸种法的结果一致。此外，田间试验进一步验证了对乙胺嘧啶和毒死rif耐药性的诊断准确性。作为一种简便易用的工具（便携式，检测时间短），S。furcifera的抗药性诊断试剂盒很容易推广。其出色的性能使该套件成为确定有效杀虫剂的可靠筛选工具，并且是综合虫害管理程序的便捷补充。