Objective and design

Biochanin A (BCA), a phytoestrogen, has various pharmacological properties. This study was conducted to compare BCA’s therapeutic property against 17-β estradiol replacement therapy in zymosan-induced arthritis (ZIA) in mice. Additionally, we further investigated in vitro the anti-inflammatory action on neutrophils.

Treatment

Ovariectomized (OVX) and non-OVX mice were pretreated with BCA (1, 3 and 9 mg/kg) or estrogen (50 µg/kg) for 14 days prior to ZIA. Neutrophils were pretreated with BCA (1, 10 and 100 μM) for 1 h prior to phorbol 12-myristate 13-acetate.

Methods

Anti-inflammatory effects of BCA were evaluated by cellular infiltrate, paw edema and cytokine measurement. In vitro, apoptosis was assessed by morphology and flow cytometry. Neutrophil extracellular traps (NET) were determined by fluorescent microscopy and DNA release. Statistical differences were determined by one- or two-way ANOVA.

Results

BCA inhibited neutrophil accumulation, paw edema and proinflammatory cytokine (TNF-α and IFN-γ) and increased anti-inflammatory cytokines (IL-4 and IL-10) in OVX and non-OVX mice, similar to 17-β estradiol replacement therapy. In vitro, BCA increased apoptosis and consequently reduced NETs.

Conclusion

BCA has a notable anti-inflammatory effect, similar to 17-β estradiol, and is especially effective for treatment of ZIA. These results suggest that BCA may be promising for the treatment of postmenopausal arthritis.

中文翻译：

---

Biochanin A与17-β雌二醇相似，可减轻小鼠酵母聚糖诱导的关节炎，这是激素替代疗法的替代方法吗？

目标和设计

生物雌激素A（BCA）是一种植物雌激素，具有多种药理特性。进行这项研究以比较BCA在酵母聚糖诱导的关节炎（ZIA）中对17-β雌二醇替代疗法的治疗作用。此外，我们进一步研究了对中性粒细胞的抗炎作用。

治疗

在ZIA之前，将BCA（1、3和9 m​​g / kg）或雌激素（50 µg / kg）预处理去卵巢（OVX）和非OVX小鼠14天。中性粒细胞用BCA（1、10和100μM）预处理1小时，然后佛波醇12-肉豆蔻酸酯13-乙酸酯。

方法

通过细胞浸润，爪水肿和细胞因子测量评估BCA的抗炎作用。在体外，通过形态学和流式细胞术评估凋亡。通过荧光显微镜和DNA释放测定嗜中性白细胞胞外陷阱（NET）。统计差异通过单向或双向方差分析确定。

结果

BCA抑制OVX和非OVX小鼠中的中性粒细胞积聚，爪水肿和促炎细胞因子（TNF-α和IFN-γ）并增加抗炎细胞因子（IL-4和IL-10），类似于17-β雌二醇替代疗法。在体外，BCA增加细胞凋亡并因此减少NETs。

结论

BCA具有明显的抗炎作用，类似于17-β雌二醇，对ZIA的治疗尤其有效。这些结果表明，BCA对于绝经后关节炎的治疗可能是有希望的。